Development and Optimization of Nanoparticles Loaded with Erucin, a Dietary Isothiocyanate Isolated from Antioxidant and Antiproliferative Activities in Ehrlich-ascites Carcinoma Cell Line

Overview

Journal Front Pharmacol

Date 2023 Feb 10

PMID 36761468

Authors

Sharabjit Singh

Gurdeep Singh

Shivani Attri

Prabhjot Kaur

Farhana Rashid

Neena Bedi

Shafiul Haque

Essam M Janahi

Saroj Arora

Affiliations

Soon will be listed here.

Abstract

The study on Erucin (ER) has gained interest of nutraceutical and pharmaceutical industries because of its anti-cancer properties. Erucin is an isothiocyanate obtained from the seeds of which possess certain drawbacks such as poor aqueous solubility and bioavailability. Therefore, the present study aimed at developing ER-cubosomes (CUB) by solvent evaporation technique followed by applying Central Composite Design to optimize ER loaded cubosomes. For this purpose, independent variables selected were Monoolein (MO) as lipid and Pluronic-84 (P-84) as a stabilizer whereas dependent variables were particle size, percentage of ER loading and percentage of its entrapment efficiency. The cubosomal nanocarriers exhibited particle size in the range of 26 nm, entrapment efficiency of 99.12 ± 0.04% and drug loading of 3.96 ± 0.0001%. Furthermore, to investigate the antioxidant potential, we checked the effect of ER and ER-CUB by DNA nicking assay, DDPH assay and Phosphomolybdate assay, and results showed significant improvement in antioxidant potential for ER-CUB than ER. Similarly, ER-CUB showed enhanced anticancer activity with a marked reduction in IC50 value than ER in MTT assay. These results suggested that ER-CUB produced notable escalation in antioxidant potential and enhanced anticancer activity than ER.

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