Propofol-mediated Circ_0000735 Downregulation Restrains Tumor Growth by Decreasing Integrin-β1 Expression in Non-small Cell Lung Cancer

Overview

Journal Open Med (Wars)

Specialty General Medicine

Date 2023 Feb 10

PMID 36760719

Authors

Lihui Zhang

Yunli Gao

Yue Li

Xinying Li

Haixia Gong

Affiliations

Soon will be listed here.

Abstract

Propofol, an intravenous anesthetic agent, exerts an anti-tumor peculiarity in multifarious tumors. Circular RNA hsa_circ_0000735 (circ_0000735) is involved in non-small cell lung cancer (NSCLC) progression. The purpose of this study is to investigate whether propofol can curb NSCLC progression via regulating circ_0000735 expression. Cell viability, proliferation, apoptosis, and invasion were detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 5-ethynyl-2'-deoxyuridine, flow cytometry, and transwell assays. Evaluation of protein levels was performed using western blotting or immunohistochemistry. Detection of circ_0000735 in tissue samples and cells was carried out using a real-time quantitative polymerase chain reaction. The molecular mechanisms associated with circ_0000735 were predicted by bioinformatics analysis and verified by dual-luciferase reporter assays. The relationship between propofol and circ_0000735 was verified by xenograft models. The results showed that circ_0000735 was overexpressed in NSCLC samples and cells. Propofol treatment overtly decreased circ_0000735 expression in NSCLC cells and repressed NSCLC cell viability, proliferation, invasion, and facilitated NSCLC cell apoptosis, but these effects mediated by propofol were counteracted by circ_0000735 overexpression. Circ_0000735 functioned as a miR-153-3p sponge and regulated integrin-β1 (ITGB1) expression via adsorbing miR-153-3p. ITGB1 overexpression reversed circ_0000735 silencing-mediated effects on NSCLC cell viability, proliferation, invasion, and apoptosis. In conclusion, propofol restrained NSCLC growth by downregulating circ_0000735, which functioned as a miR-153-3p sponge and regulated ITGB1 expression via adsorbing miR-153-3p. This study provides evidence to support that propofol curbs NSCLC progression by regulating circRNA expression.

References

Huang W, Xu X, Liu M, Cui W, Peng G . Downregulation of Hsa_circ_0000735 Inhibits the Proliferation, Migration, Invasion, and Glycolysis in Non-small-cell Lung Cancer by Targeting miR-940/BMPER Axis. Onco Targets Ther. 2020; 13:8427-8439. PMC: 7457839. DOI: 10.2147/OTT.S253474. View

Jiang S, Liu Y, Huang L, Zhang F, Kang R . Effects of propofol on cancer development and chemotherapy: Potential mechanisms. Eur J Pharmacol. 2018; 831:46-51. DOI: 10.1016/j.ejphar.2018.04.009. View

Ma H, Tian T, Liu X, Xia M, Chen C, Mai L . Upregulated circ_0005576 facilitates cervical cancer progression via the miR-153/KIF20A axis. Biomed Pharmacother. 2019; 118:109311. DOI: 10.1016/j.biopha.2019.109311. View

Li Y, Sun C, Tan Y, Zhang H, Li Y, Zou H . ITGB1 enhances the Radioresistance of human Non-small Cell Lung Cancer Cells by modulating the DNA damage response and YAP1-induced Epithelial-mesenchymal Transition. Int J Biol Sci. 2021; 17(2):635-650. PMC: 7893583. DOI: 10.7150/ijbs.52319. View

Lei B, Tian Z, Fan W, Ni B . Circular RNA: a novel biomarker and therapeutic target for human cancers. Int J Med Sci. 2019; 16(2):292-301. PMC: 6367529. DOI: 10.7150/ijms.28047. View

Sui H, Zhu C, Li Z, Yang J . Propofol suppresses gastric cancer tumorigenesis by modulating the circular RNA‑PVT1/miR‑195‑5p/E26 oncogene homolog 1 axis. Oncol Rep. 2020; 44(4):1736-1746. DOI: 10.3892/or.2020.7725. View

Hynes R . Integrins: bidirectional, allosteric signaling machines. Cell. 2002; 110(6):673-87. DOI: 10.1016/s0092-8674(02)00971-6. View

Zhang C, Wu S, Song R, Liu C . Long noncoding RNA NR2F1-AS1 promotes the malignancy of non-small cell lung cancer via sponging microRNA-493-5p and thereby increasing ITGB1 expression. Aging (Albany NY). 2020; 13(5):7660-7675. PMC: 7993723. DOI: 10.18632/aging.103564. View

Zheng H, Yang C, Tang J . Cyclic RNA Circ_0000735 sponges miR-502-5p to promote bladder cancer cell proliferation and invasion and inhibit apoptosis. Int J Clin Exp Pathol. 2021; 13(12):2994-3003. PMC: 7791388. View

10.

Sun Q, Zhou C, Ma R, Guo Q, Huang H, Hao J . Prognostic value of increased integrin-beta 1 expression in solid cancers: a meta-analysis. Onco Targets Ther. 2018; 11:1787-1799. PMC: 5881529. DOI: 10.2147/OTT.S155279. View

11.

Xu X, Zhang X, Zhang Y, Wang Z . Curcumin suppresses the malignancy of non-small cell lung cancer by modulating the circ-PRKCA/miR-384/ITGB1 pathway. Biomed Pharmacother. 2021; 138:111439. DOI: 10.1016/j.biopha.2021.111439. View

12.

Yap A, Lopez-Olivo M, Dubowitz J, Hiller J, Riedel B . Anesthetic technique and cancer outcomes: a meta-analysis of total intravenous versus volatile anesthesia. Can J Anaesth. 2019; 66(5):546-561. DOI: 10.1007/s12630-019-01330-x. View

13.

Li R, Liu H, Dilger J, Lin J . Effect of Propofol on breast Cancer cell, the immune system, and patient outcome. BMC Anesthesiol. 2018; 18(1):77. PMC: 6020422. DOI: 10.1186/s12871-018-0543-3. View

14.

Xu Z, Zhang M, Chen S, Qiu M, Zhang Q, Gao L . MicroRNA-424-5p inhibits the development of non-small cell LCa by binding to ITGB1. Eur Rev Med Pharmacol Sci. 2019; 23(20):8921-8930. DOI: 10.26355/eurrev_201910_19289. View

15.

Li W, Jiang W, Liu T, Lv J, Guan J . Enhanced expression of circ_0000735 forecasts clinical severity in NSCLC and promotes cell progression via sponging miR-1179 and miR-1182. Biochem Biophys Res Commun. 2019; 510(3):467-471. DOI: 10.1016/j.bbrc.2019.01.134. View

16.

Cui X, Feng J, Wu J, Zhang X, Ding M . Propofol postpones colorectal cancer development through circ_0026344/miR-645/Akt/mTOR signal pathway. Open Med (Wars). 2021; 16(1):570-580. PMC: 8034241. DOI: 10.1515/med-2021-0254. View

17.

Zhao G, Zhang Y, Zhao Z, Cai H, Zhao X, Yang T . MiR-153 reduces stem cell-like phenotype and tumor growth of lung adenocarcinoma by targeting Jagged1. Stem Cell Res Ther. 2020; 11(1):170. PMC: 7201619. DOI: 10.1186/s13287-020-01679-7. View

18.

Qin Q, Wei F, Zhang J, Li B . miR-134 suppresses the migration and invasion of non‑small cell lung cancer by targeting ITGB1. Oncol Rep. 2017; 37(2):823-830. DOI: 10.3892/or.2017.5350. View

19.

Gao Y, Liu J, Huan J, Che F . Downregulation of circular RNA hsa_circ_0000735 boosts prostate cancer sensitivity to docetaxel via sponging miR-7. Cancer Cell Int. 2020; 20:334. PMC: 7376840. DOI: 10.1186/s12935-020-01421-6. View

20.

Gao J, Ding C, Zhou J, Wu G, Han Z, Li J . Propofol suppresses lung cancer tumorigenesis by modulating the circ-ERBB2/miR-7-5p/FOXM1 axis. Thorac Cancer. 2021; 12(6):824-834. PMC: 7952809. DOI: 10.1111/1759-7714.13856. View