Colonized Patients by : Third and Largest Outbreak in Brazil and Impact of Biofilm Formation

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2023 Feb 9

PMID 36756619

Authors

Camylla Carvalho de Melo

Bruna Rodrigues de Sousa

Gisela Lara da Costa

Manoel Marques Evangelista Oliveira

Reginaldo Goncalves de Lima-Neto

Affiliations

Soon will be listed here.

Abstract

Objective: To describe the clinical-epidemiological features of patients colonized by in the largest outbreak in Brazil and to show the biofilm formation capacity of yeast strains.

Methods: Clinical yeasts suspected of isolated from urine and surveillance samples were seeded on chromogenic media at 30°C and Sabouraud agar at 42°C. matrix-assisted laser desorption/ionization-time of flight mass spectometry was used for reliable identification. After proteomic confirmation, the genomic approach and culture on Chromagar Plus media were carried out. Biofilm formation was investigated based on metabolic activity, and the clinical-epidemiological profile of patients was described.

Results: A total of 11 C clinical yeasts from nine patients were identified between the end of December 2021 and March 2022. Two clinical yeasts were isolates from urine and nine clinical yeasts were isolates from axillary and inguinal surveillance swabs. No case is related to previous Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, all the yeasts showed a high ability of biofilm formation.

Conclusion: requires great vigilance as its high capacity to colonize and form biofilms contributes to its dissemination. The rapid and precise identification of this species is essential for the management, control, and prevention of infections.

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References

Pierce C, Uppuluri P, Tristan A, Wormley Jr F, Mowat E, Ramage G . A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc. 2008; 3(9):1494-500. PMC: 2741160. DOI: 10.1038/nport.2008.141. View

Lockhart S, Etienne K, Vallabhaneni S, Farooqi J, Chowdhary A, Govender N . Simultaneous Emergence of Multidrug-Resistant Candida auris on 3 Continents Confirmed by Whole-Genome Sequencing and Epidemiological Analyses. Clin Infect Dis. 2016; 64(2):134-140. PMC: 5215215. DOI: 10.1093/cid/ciw691. View

Aldejohann A, Wiese-Posselt M, Gastmeier P, Kurzai O . Expert recommendations for prevention and management of Candida auris transmission. Mycoses. 2022; 65(6):590-598. DOI: 10.1111/myc.13445. View

Mizusawa M, Miller H, Green R, Lee R, Durante M, Perkins R . Can Multidrug-Resistant Candida auris Be Reliably Identified in Clinical Microbiology Laboratories?. J Clin Microbiol. 2016; 55(2):638-640. PMC: 5277535. DOI: 10.1128/JCM.02202-16. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

de Almeida Jr J, Francisco E, Hagen F, Brandao I, Pereira F, Dias P . Emergence of in Brazil in a COVID-19 Intensive Care Unit. J Fungi (Basel). 2021; 7(3). PMC: 8002986. DOI: 10.3390/jof7030220. View

Satoh K, Makimura K, Hasumi Y, Nishiyama Y, Uchida K, Yamaguchi H . Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital. Microbiol Immunol. 2009; 53(1):41-4. DOI: 10.1111/j.1348-0421.2008.00083.x. View

Briano F, Magnasco L, Sepulcri C, Dettori S, Dentone C, Mikulska M . Candida auris Candidemia in Critically Ill, Colonized Patients: Cumulative Incidence and Risk Factors. Infect Dis Ther. 2022; 11(3):1149-1160. PMC: 8995918. DOI: 10.1007/s40121-022-00625-9. View

Lindsley M, Hurst S, Iqbal N, Morrison C . Rapid identification of dimorphic and yeast-like fungal pathogens using specific DNA probes. J Clin Microbiol. 2001; 39(10):3505-11. PMC: 88380. DOI: 10.1128/JCM.39.10.3505-3511.2001. View

10.

Sabino R, Verissimo C, Pereira A, Antunes F . , an Agent of Hospital-Associated Outbreaks: Which Challenging Issues Do We Need to Have in Mind?. Microorganisms. 2020; 8(2). PMC: 7074697. DOI: 10.3390/microorganisms8020181. View

11.

Horton M, Nett J . infection and biofilm formation: going beyond the surface. Curr Clin Microbiol Rep. 2020; 7(3):51-56. PMC: 7654955. DOI: 10.1007/s40588-020-00143-7. View

12.

Freimoser F, JAKOB C, Aebi M, Tuor U . The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities. Appl Environ Microbiol. 1999; 65(8):3727-9. PMC: 91559. DOI: 10.1128/AEM.65.8.3727-3729.1999. View

13.

Zhu Y, OBrien B, Leach L, Clarke A, Bates M, Adams E . Laboratory Analysis of an Outbreak of Candida auris in New York from 2016 to 2018: Impact and Lessons Learned. J Clin Microbiol. 2019; 58(4). PMC: 7098748. DOI: 10.1128/JCM.01503-19. View

14.

Singh R, Kaur M, Chakrabarti A, Shankarnarayan S, Rudramurthy S . Biofilm formation by Candida auris isolated from colonising sites and candidemia cases. Mycoses. 2019; 62(8):706-709. DOI: 10.1111/myc.12947. View

15.

Rossato L, Colombo A . What Have We Learned About Its Mechanisms of Pathogenicity?. Front Microbiol. 2019; 9:3081. PMC: 6315175. DOI: 10.3389/fmicb.2018.03081. View

16.

Keighley C, Garnham K, Harch S, Robertson M, Chaw K, Teng J . Diagnostic Challenges and Emerging Opportunities for the Clinical Microbiology Laboratory. Curr Fungal Infect Rep. 2021; 15(3):116-126. PMC: 8220427. DOI: 10.1007/s12281-021-00420-y. View

17.

Krom B, Cohen J, McElhaney Feser G, Cihlar R . Optimized candidal biofilm microtiter assay. J Microbiol Methods. 2006; 68(2):421-3. DOI: 10.1016/j.mimet.2006.08.003. View

18.

Berridge M, Herst P, Tan A . Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005; 11:127-52. DOI: 10.1016/S1387-2656(05)11004-7. View

19.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

20.

Kean R, Mckloud E, Townsend E, Sherry L, Delaney C, Jones B . The comparative efficacy of antiseptics against Candida auris biofilms. Int J Antimicrob Agents. 2018; 52(5):673-677. DOI: 10.1016/j.ijantimicag.2018.05.007. View