Ultra-thin Membrane Filter with a Uniformly Arrayed Nanopore Structure for Nanoscale Separation of Extracellular Vesicles Without Cake Formation

Overview

Journal Nanoscale Adv

Publisher Royal Society of Chemistry

Specialty Biotechnology

Date 2023 Feb 9

PMID 36756507

Authors

Daesan Kim

Jaehyuk Lee

Boyoung Kim

YuJin Shin

Jinhong Park

Uijoo Kim

Minbaek Lee

Sang Bum Kim

Sunghoon Kim

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) have emerged as vehicles that mediate diverse cell-cell communication. However, in-depth understanding of these vesicles is hampered by a lack of a reliable isolation method to separate different types of EVs with high levels of integrity and purity. Here, we developed a nanoporous and ultra-thin membrane structure (NUTS) that warrants the size-based isolation of EVs without cake formation, minimizing the sample loss during the filtration process. By utilizing the micro-electro-mechanical systems (MEMS) technique, we could also control the pore size in nanoscale. We validated the performance of this membrane to separate EVs according to their size range.

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