Loss of Pten in Renal Tubular Cells Leads to Water Retention by Upregulating AQP2

Overview

Journal Kidney Dis (Basel)

Specialty Nephrology

Date 2023 Feb 9

PMID 36756085

Authors

Zhuo Sun

Xiaotong Shao

Haotian Wu

Yaxian Zhao

Yidan Cao

Danhua Li

Ying Sun

Qingling Wang

Affiliations

Soon will be listed here.

Abstract

Introduction: Phosphatase and tensin (PTEN) is a multifunctional gene associated with the normal development and physiological function of various tissues including the kidney. However, its role in renal tubular reabsorption function has not been well elucidated.

Methods: We generated a renal tubule-specific knockout mouse model by crossing mice with transgenic mice, evaluated the effect of loss on renal tubular function, and investigated the underlying mechanisms.

Results: loss resulted in abnormal renal structure and function and water retention in multiple organs. Our results also demonstrated that aquaporin-2 (AQP2), an important water channel protein, was upregulated and concentrated on the apical plasma membrane of collecting duct cells, which could be responsible for the impaired water balance in loss mice. The regulation of loss on AQP2 was mediated by protein kinase B (AKT) activation.

Conclusions: Our results reveal a connection between gene inactivation and water retention, suggesting the importance of PTEN in normal kidney development and function.

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