Hyperbaric Oxygen Therapy Ameliorates Sperm Parameters in Apolipoprotein E Knockout Mice Testes by Attenuating Oxidative Stress and Inflammation

Overview

Journal Reprod Sci

Publisher Springer

Specialty Reproductive Medicine

Date 2023 Feb 6

PMID 36745359

Authors

Huijun Liu

Danni Wang

Yang Ma

Huiting Sun

Linxiao Wang

Yichao Shi

Jiaping Wang

Xia Chen

Affiliations

Soon will be listed here.

Abstract

Apolipoprotein E (ApoE) is a member of apolipoprotein (apo) family and plays critical role in lipid metabolism. In this study, the relationship between abnormal lipid metabolism caused by ApoE-deficient and male reproduction was investigated. The effect of hyperbaric oxygen (HBO) therapy on 7-month-old ApoE-knockout male mice was assessed subsequently. Mice were randomly divided into 3 groups: control group (WT), ApoE (- / -) group (AP-CON), and ApoE (- / -) plus HBO group (AP-HBO), which received HBO treatment. We found that ApoE knockout caused a decrease in male reproductive capacity due to the reduced total sperm motility, progressive motility (PR), and lower blastocyst formation rate. HBO treatment could accelerate serum lipoprotein metabolism including LDL, T-CHO, and TG and semen quality. As a result, fertilization and blastocyst formation of AP-HBO group were higher than that of AP-CON, proving positive therapeutic effect. Mechanism exploration found that HBO treatment ameliorated the testicular microenvironment by attenuating inflammatory factor production and oxidative stress, eventually improved the sperm motility. Collectively, our study provided more evidences of HBO treatment for improving the semen quality of patients with abnormal lipid metabolism caused by ApoE-deficient.

Citing Articles

Proteomic Landscape of Human Sperm in Patients with Different Spermatogenic Impairments.

Becker L, Smadi M, Raeschle M, Rishik S, Abdul-Khaliq H, Meese E Cells. 2023; 12(7).

PMID: 37048090 PMC: 10093380. DOI: 10.3390/cells12071017.

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