Multiomics Integration Reveals the Effect of Orexin A on Glioblastoma

Overview

Journal Front Pharmacol

Date 2023 Feb 6

PMID 36744263

Authors

Sha Yang

Renzheng Huan

Jianhe Yue

Jin Guo

Mei Deng

Liya Wang

Shuo Peng

Xin Lin

Lin Liu

Jia Wang

Guoqiang Han

Yan Zha

Jian Liu

Jiqin Zhang

Ying Tan

Affiliations

Soon will be listed here.

Abstract

This study involved a multi-omics analysis of glioblastoma (GBM) samples to elaborate the potential mechanism of drug treatment. The GBM cells treated with or without orexin A were acquired from sequencing analysis. Differentially expressed genes/proteins/metabolites (DEGs/ DEPs/ DEMs) were screened. Next, combination analyses were conducted to investigate the common pathways and correlations between the two groups. Lastly, transcriptome-proteome-metabolome association analysis was carried out to determine the common pathways, and the genes in these pathways were analyzed through Kaplan-Meier (K-M) survival analysis in public databases. Cell and animal experiments were performed to investigate the anti-glioma activity of orexin A. A total of 1,527 DEGs, 52 DEPs, and 153 DEMs were found. Moreover, the combination analyses revealed that 6, 4, and 1 common pathways were present in the transcriptome-proteome, proteome-metabolome, and transcriptome-metabolome, respectively. Certain correlations were observed between the two data sets. Finally, 11 common pathways were discovered in association analysis, and 138 common genes were screened out in these common pathways. Six genes showed significant differences in terms of survival in both TCGA and CGGA. In addition, orexin A inhibited the proliferation, migration, and invasion of glioma and . Eleven common KEGG pathways with six common genes were found among different omics participations, revealing the underlying mechanisms in different omics and providing theoretical basis and reference for multi-omics research on drug treatment.

Citing Articles

Orexin-A mediates glioblastoma proliferation inhibition by increasing ferroptosis triggered by unstable iron pools and GPX4 depletion.

Huan R, Zhang J, Yue J, Yang S, Han G, Cheng Y J Cell Mol Med. 2024; 28(9):e18318.

PMID: 38685674 PMC: 11058333. DOI: 10.1111/jcmm.18318.

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