Expressions and Prognostic Values of Notch3 and DLL4 in Human Breast Cancer

Overview

Journal Technol Cancer Res Treat

Specialties Biomedical Engineering
Oncology
Pharmacology

Date 2023 Feb 6

PMID 36740988

Authors

Lin Wang

Ge Cao

Wei Li

Xiao-Wen Chen

Shi-Shuang Xiong

Ya-Nan Mu

Jin-Fang Jiang

Lan Yang

De-Rui Zhang

Yu-Wen Cao

Affiliations

Soon will be listed here.

Abstract

: Notch signaling played a critical role in promoting breast tumorigenesis and progression. However, the role and prognostic value of Notch3 combined with DLL4 expression in breast carcinoma had not been explored. The retrospective study enrolled 90 breast cancer tissues and 60 noncancerous tissues from (conceal). The expression and prognostic value of Notch3 and DLL4 in patients with breast carcinoma were investigated using Oncomine and UALCAN database. Notch3 and DLL4 expression levels were detected by quantitative real-time polymerase chain reaction, western blotting, and immunohistochemistry. We analyzed the correlation between both proteins expression and clinicopathological parameters and survival data, respectively. The expressions of Notch3 and DLL4 were increased, and Notch3 expression was significantly positively associated with DLL4 in breast carcinoma. The 2 proteins dramatically correlated with advanced stage, high grade and negative Her2 status. The overexpressing of single or both Notch3 and DLL4 resulted in shortened survival of breast cancer patients. And Notch3 overexpression was one of independent risk predictors to poor prognosis. The interaction of Notch3 receptor and DLL4 ligand accelerates oncogenesis, progression, and poor prognosis of breast cancer patients. Notch3 protein may serve as one of biomarker to independently predict prognosis of patients.

Citing Articles

Notch2/3-DLL4 interaction in urothelial cancer cell lines supports a tumorigenic role of Notch signaling pathways in bladder carcinoma.

Zhang C, Weimann A, Stolzenburg J, Neuhaus J, Berndt-Paetz M PLoS One. 2025; 20(2):e0317709.

PMID: 39951484 PMC: 11828355. DOI: 10.1371/journal.pone.0317709.

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