Purification and Biochemical Characterization of a Novel Ene- Reductase from Kazachstania Exigua HSC6 for Dihydro-β-ionone from β-ionone

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2023 Feb 4

PMID 36738355

Authors

Zhangde Long

Kena Li

Yun Xue

Yongwei Sun

Jigang Li

Zan Su

Jiansheng Sun

Qibin Liu

Hong Liu

Tao Wei

Affiliations

Soon will be listed here.

Abstract

Purpose: We purified and characterized a novel ene-reductase (KaDBR1) from Kazachstania exigua HSC6 for the synthesis of dihydro-β-ionone from β-ionone.

Methods: KaDBR1 was purified to homogeneity by ammonium sulfate precipitation and phenyl-Sepharose Fast Flow and Q-Sepharose chromatography. The purified enzyme was characterized by measuring the amount of dihydro-β-ionone from β-ionone with LC-MS analysis method.

Results: The molecular mass of KaDBR1 was estimated to be 45 kDa by SDS-PAGE. The purified KaDBR1 enzyme had optimal activity at 60 °C and pH 6.0. The addition of 5 mM Mg, Ca, Al, Na, and dithiothreitol increased the activity of KaDBR1 by 25%, 18%, 34%, 20%, and 23%, respectively. KaDBR1 favored NADH over NADPH as a cofactor, and its catalytic efficiency (kcat/Km) toward β-ionone using NADH was 8.1-fold greater than when using NADPH.

Conclusion: Owing to its unique properties, KaDBR1 is a potential candidate for the enzymatic biotransformation of β-ionone to dihydro-β-ionone in biotechnology applications.

Citing Articles

Applications of Ene-Reductases in the Synthesis of Flavors and Fragrances.

Fan X, Yu Y, Yao Y, Li W, Tao F, Wang N J Agric Food Chem. 2024; 72(33):18305-18320.

PMID: 38966982 PMC: 11342376. DOI: 10.1021/acs.jafc.4c02897.

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