Rice Lipid Transfer Protein, OsLTPL23, Controls Seed Germination by Regulating Starch-sugar Conversion and ABA Homeostasis

Overview

Journal Front Genet

Date 2023 Feb 2

PMID 36726806

Authors

Quanlin Li

Wenxue Zhai

Jiaping Wei

Yanfeng Jia

Affiliations

Soon will be listed here.

Abstract

Seed germination is vital for ensuring the continuity of life in spermatophyte. High-quality seed germination usually represents good seedling establishment and plant production. Here, we identified OsLTPL23, a putative rice non-specific lipid transport protein, as an important regulator responsible for seed germination. Subcellular localization analysis confirmed that OsLTPL23 is present in the plasma membrane and nucleus. The knockout mutants of were generated by CRISPR/Cas9-mediated genome editing, and lines significantly germinated slower and lower than the Nipponbare (NIP). Starch and soluble sugar contents measurement showed that OsLTPL23 may have alpha-amylase inhibitor activity, and high soluble sugar content may be a causal agent for the delayed seed germination of mutants. Transcript profiles in the germinating seeds exhibited that the abscisic acid (ABA)-responsive genes, and , and biosynthesis genes, , , and , are obviously upregulated in the mutants compared to NIP plants, conversely, ABA metabolism genes , and are stepwise decreased. Further investigations found that mutants displays weakened early seedling growth, with elevated gene expresssion of ABA catabolism genes and repressive transcription response of defence-related genes , and . Integrated analysis indicated that may exert an favorable effect on rice seed germination and early seedling growth modulating endogenous ABA homeostasis. Collectively, our study provides important insights into the roles of -mediated carbohydrate conversion and endogenous ABA pathway on seed germination and early seedling growth, which contributes to high-vigor seed production in rice breeding.

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References

Penfield S . Seed dormancy and germination. Curr Biol. 2017; 27(17):R874-R878. DOI: 10.1016/j.cub.2017.05.050. View

Zhu G, Ye N, Zhang J . Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis. Plant Cell Physiol. 2009; 50(3):644-51. DOI: 10.1093/pcp/pcp022. View

Shu K, Zhang H, Wang S, Chen M, Wu Y, Tang S . ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in arabidopsis. PLoS Genet. 2013; 9(6):e1003577. PMC: 3688486. DOI: 10.1371/journal.pgen.1003577. View

Meng C, Yan Y, Liu Z, Chen L, Zhang Y, Li X . Systematic Analysis of Cotton Non-specific Lipid Transfer Protein Family Revealed a Special Group That Is Involved in Fiber Elongation. Front Plant Sci. 2018; 9:1285. PMC: 6156462. DOI: 10.3389/fpls.2018.01285. View

Wang Z, Wang J, Bao Y, Wang F, Zhang H . Quantitative trait loci analysis for rice seed vigor during the germination stage. J Zhejiang Univ Sci B. 2010; 11(12):958-64. PMC: 2997405. DOI: 10.1631/jzus.B1000238. View

Finch-Savage W, Bassel G . Seed vigour and crop establishment: extending performance beyond adaptation. J Exp Bot. 2015; 67(3):567-91. DOI: 10.1093/jxb/erv490. View

Tao Y, Zou T, Zhang X, Liu R, Chen H, Yuan G . Secretory lipid transfer protein OsLTPL94 acts as a target of EAT1 and is required for rice pollen wall development. Plant J. 2021; 108(2):358-377. DOI: 10.1111/tpj.15443. View

CUTLER S, Ehrhardt D, Griffitts J, Somerville C . Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency. Proc Natl Acad Sci U S A. 2000; 97(7):3718-23. PMC: 16306. DOI: 10.1073/pnas.97.7.3718. View

Jacobsen J, Pearce D, Poole A, Pharis R, Mander L . Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley. Physiol Plant. 2002; 115(3):428-441. DOI: 10.1034/j.1399-3054.2002.1150313.x. View

10.

Yang L, Liu S, Lin R . The role of light in regulating seed dormancy and germination. J Integr Plant Biol. 2020; 62(9):1310-1326. DOI: 10.1111/jipb.13001. View

11.

Zhang D, Liang W, Yin C, Zong J, Gu F, Zhang D . OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiol. 2010; 154(1):149-62. PMC: 2938136. DOI: 10.1104/pp.110.158865. View

12.

Fleury C, Gracy J, Gautier M, Pons J, Dufayard J, Labesse G . Comprehensive classification of the plant non-specific lipid transfer protein superfamily towards its sequence-structure-function analysis. PeerJ. 2019; 7:e7504. PMC: 6698131. DOI: 10.7717/peerj.7504. View

13.

Matsukura C, Saitoh T, Hirose T, Ohsugi R, Perata P, Yamaguchi J . Sugar uptake and transport in rice embryo. Expression of companion cell-specific sucrose transporter (OsSUT1) induced by sugar and light. Plant Physiol. 2000; 124(1):85-93. PMC: 59124. DOI: 10.1104/pp.124.1.85. View

14.

Galland M, Boutet-Mercey S, Lounifi I, Godin B, Balzergue S, Grandjean O . Compartmentation and dynamics of flavone metabolism in dry and germinated rice seeds. Plant Cell Physiol. 2014; 55(9):1646-59. DOI: 10.1093/pcp/pcu095. View

15.

Preston J, Tatematsu K, Kanno Y, Hobo T, Kimura M, Jikumaru Y . Temporal expression patterns of hormone metabolism genes during imbibition of Arabidopsis thaliana seeds: a comparative study on dormant and non-dormant accessions. Plant Cell Physiol. 2009; 50(10):1786-800. DOI: 10.1093/pcp/pcp121. View

16.

Kader J . LIPID-TRANSFER PROTEINS IN PLANTS. Annu Rev Plant Physiol Plant Mol Biol. 1996; 47:627-654. DOI: 10.1146/annurev.arplant.47.1.627. View

17.

Luo X, Dai Y, Zheng C, Yang Y, Chen W, Wang Q . The ABI4-RbohD/VTC2 regulatory module promotes reactive oxygen species (ROS) accumulation to decrease seed germination under salinity stress. New Phytol. 2020; 229(2):950-962. DOI: 10.1111/nph.16921. View

18.

Rajjou L, Duval M, Gallardo K, Catusse J, Bally J, Job C . Seed germination and vigor. Annu Rev Plant Biol. 2011; 63:507-33. DOI: 10.1146/annurev-arplant-042811-105550. View

19.

Molina A, Garcia-Olmedo F . Enhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2. Plant J. 1997; 12(3):669-75. DOI: 10.1046/j.1365-313x.1997.00669.x. View

20.

Deng W, Li R, Xu Y, Mao R, Chen S, Chen L . A lipid transfer protein variant with a mutant eight-cysteine motif causes photoperiod- and thermo-sensitive dwarfism in rice. J Exp Bot. 2019; 71(4):1294-1305. PMC: 7031082. DOI: 10.1093/jxb/erz500. View