Time-dependent Microbial Shifts During Crayfish Decomposition in Freshwater and Sediment Under Different Environmental Conditions

Overview

Journal Sci Rep

Specialty Science

Date 2023 Jan 27

PMID 36707669

Authors

Bastian Mahler

Kathrin Janssen

Mara Iris Lonartz

Markus Lagos

Thorsten Geisler

Jes Rust

Gabriele Bierbaum

Affiliations

Soon will be listed here.

Abstract

Fossilization processes and especially the role of bacterial activity during the preservation of organic material has not yet been well understood. Here, we report the results of controlled taphonomic experiments with crayfish in freshwater and sediment. 16S rRNA amplicon analyzes showed that the development of the bacterial community composition over time was correlated with different stages of decay and preservation. Three dominating genera, Aeromonas, Clostridium and Acetobacteroides were identified as the main drivers in the decomposition of crayfish in freshwater. Using micro-computed tomography (µ-CT), scanning electron microscopy (SEM) and confocal Raman spectroscopy (CRS), calcite clusters were detected after 3-4 days inside crayfish carcasses during their decomposition in freshwater at 24 °C. The precipitation of calcite clusters during the decomposition process was increased in the presence of the bacterial genus Proteocatella. Consequently, Proteocatella might be one of the bacterial genera responsible for fossilization.

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