Antibacterial Activity and Mechanism of Flavonoids from S. Y. Hu. and Its Transcriptome Analysis Against

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Jan 27

PMID 36704556

Authors

Huan Zhou

Lingli Chen

Kehui Ouyang

Qingfeng Zhang

Wenjun Wang

Affiliations

Soon will be listed here.

Abstract

Introduction: S. Y. Hu. (FCS) possess many biological activities, but the antibacterial activity and underlying mechanisms of flavonoids from Chimonanthus salicifolius S. Y. Hu. (FCS) is still unknown.

Method: Maximum diameter of inhibition zone (DIZ), maximum diameter of inhibition zone (DIZ), the lowest minimum inhibition concentration (MIC), and the lowest minimum bactericide concentration (MBC) were used to detect the antibacterial activity. Meanwhile, related enzyme activities, the transcriptome analysis and quantitative RT-PCR were used to investigate the antibacterial activity mechanisms.

Results: The results showed that FCS (with a purity of 84.2 ± 2.0%) has potential effects on tested strains with the maximum diameter of inhibition zone (DIZ) was 15.93 ± 2.63 mm, the lowest minimum inhibition concentration (MIC) was 1.56 mg/ml and the lowest minimum bactericide concentration (MBC) was 6.25 mg/ml. In addition, the bacterial growth curve test, release of extracellular alkaline phosphatase (AKP), loss of intracellular components, DNA damage and transmission electron microscope (TEM) suggested that FCS could destroy the cell wall and membrane, cause the loss of intracellular substance, cause DNA damage and even lead to cell death. Moreover, the antibacterial mechanism of FCS against (, Gram-positive bacteria) was further confirmed by the transcriptome analysis and quantitative RT-PCR at the molecular level for the first time. A total of 671 differentially expressed genes (DEGs) were identified after treated with FCS (1/2 MIC), with 338 and 333 genes showing up-regulation and down-regulation, respectively. The highlighted changes were those related to the biosynthesis of bacteria wall and membrane, DNA replication and repair, and energy metabolism.

Discussion: Overall, our research provides theoretical guidance for the application of FCS, which is expected to be potentially used as a natural antimicrobial agent in food safety.

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References

Hiron A, Borezee-Durant E, Piard J, Juillard V . Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus. J Bacteriol. 2007; 189(14):5119-29. PMC: 1951871. DOI: 10.1128/JB.00274-07. View

Xiu L, Fu Y, Deng Y, Shi X, Bian Z, Ruhan A . Deep sequencing-based analysis of gene expression in bovine mammary epithelial cells after Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae infection. Genet Mol Res. 2015; 14(4):16948-65. DOI: 10.4238/2015.December.15.1. View

Zhang Z, Yang L, Hou J, Tian S, Liu Y . Molecular mechanisms underlying the anticancer activities of licorice flavonoids. J Ethnopharmacol. 2020; 267:113635. DOI: 10.1016/j.jep.2020.113635. View

Sun D, Zhang W, Mou Z, Chen Y, Guo F, Yang E . Transcriptome Analysis Reveals Silver Nanoparticle-Decorated Quercetin Antibacterial Molecular Mechanism. ACS Appl Mater Interfaces. 2017; 9(11):10047-10060. DOI: 10.1021/acsami.7b02380. View

Lansdown A . A pharmacological and toxicological profile of silver as an antimicrobial agent in medical devices. Adv Pharmacol Sci. 2010; 2010:910686. PMC: 3003978. DOI: 10.1155/2010/910686. View

Lin Z, Lin Y, Zhang Z, Shen J, Yang C, Jiang M . Systematic analysis of bacteriostatic mechanism of flavonoids using transcriptome and its therapeutic effect on vaginitis. Aging (Albany NY). 2020; 12(7):6292-6305. PMC: 7185132. DOI: 10.18632/aging.103024. View

Xu Z, Li H, Qin X, Wang T, Hao J, Zhao J . Antibacterial evaluation of plants extracts against ampicillin-resistant Escherichia coli (E. coli) by microcalorimetry and principal component analysis. AMB Express. 2019; 9(1):101. PMC: 6624225. DOI: 10.1186/s13568-019-0829-y. View

Yuan G, Guan Y, Yi H, Lai S, Sun Y, Cao S . Antibacterial activity and mechanism of plant flavonoids to gram-positive bacteria predicted from their lipophilicities. Sci Rep. 2021; 11(1):10471. PMC: 8131645. DOI: 10.1038/s41598-021-90035-7. View

Fathima A, Rao J . Selective toxicity of Catechin-a natural flavonoid towards bacteria. Appl Microbiol Biotechnol. 2016; 100(14):6395-6402. DOI: 10.1007/s00253-016-7492-x. View

10.

Wang J, Chi Z, Zhao K, Wang H, Zhang X, Xu F . A transcriptome analysis of the antibacterial mechanism of flavonoids from Sedum aizoon L. against Shewanella putrefaciens. World J Microbiol Biotechnol. 2020; 36(7):94. DOI: 10.1007/s11274-020-02871-w. View

11.

Ning Y, Yan A, Yang K, Wang Z, Li X, Jia Y . Antibacterial activity of phenyllactic acid against Listeria monocytogenes and Escherichia coli by dual mechanisms. Food Chem. 2017; 228:533-540. DOI: 10.1016/j.foodchem.2017.01.112. View

12.

Wang L, Zhang Z, Zeng X, Gong D, Wang M . Combination of microbiological, spectroscopic and molecular docking techniques to study the antibacterial mechanism of thymol against Staphylococcus aureus: membrane damage and genomic DNA binding. Anal Bioanal Chem. 2016; 409(6):1615-1625. DOI: 10.1007/s00216-016-0102-z. View

13.

Fleming-Jones M, Smith R . Volatile organic compounds in foods: a five year study. J Agric Food Chem. 2003; 51(27):8120-7. DOI: 10.1021/jf0303159. View

14.

Joshi S, Howell A, DSouza D . Cronobacter sakazakii reduction by blueberry proanthocyanidins. Food Microbiol. 2014; 39:127-31. DOI: 10.1016/j.fm.2013.11.002. View

15.

Chen Y, Cheng L, Zhang X, Cao J, Wu Z, Zheng X . Transcriptomic and proteomic effects of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3"Me) treatment on ethanol-stressed Saccharomyces cerevisiae cells. Food Res Int. 2019; 119:67-75. DOI: 10.1016/j.foodres.2019.01.061. View

16.

Utaida S, Dunman P, Macapagal D, Murphy E, Projan S, Singh V . Genome-wide transcriptional profiling of the response of Staphylococcus aureus to cell-wall-active antibiotics reveals a cell-wall-stress stimulon. Microbiology (Reading). 2003; 149(Pt 10):2719-2732. DOI: 10.1099/mic.0.26426-0. View

17.

Valkenburg J, Woldringh C, Brakenhoff G, van der Voort H, Nanninga N . Confocal scanning light microscopy of the Escherichia coli nucleoid: comparison with phase-contrast and electron microscope images. J Bacteriol. 1985; 161(2):478-83. PMC: 214907. DOI: 10.1128/jb.161.2.478-483.1985. View

18.

Simard F, Gauthier C, Legault J, Lavoie S, Mshvildadze V, Pichette A . Structure elucidation of anti-methicillin resistant Staphylococcus aureus (MRSA) flavonoids from balsam poplar buds. Bioorg Med Chem. 2016; 24(18):4188-4198. DOI: 10.1016/j.bmc.2016.07.009. View

19.

Sun Q, Zhu J, Cao F, Chen F . Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf. PLoS One. 2017; 12(7):e0181094. PMC: 5507308. DOI: 10.1371/journal.pone.0181094. View

20.

Wen Y, Li W, Su R, Yang M, Zhang N, Li X . Multi-Target Antibacterial Mechanism of Moringin From Seeds Against . Front Microbiol. 2022; 13:925291. PMC: 9213813. DOI: 10.3389/fmicb.2022.925291. View