Gene Regulation in Activated Microglia by Adenosine A Receptor Agonists: a Transcriptomics Study

Overview

Journal Purinergic Signal

Publisher Springer

Date 2023 Jan 26

PMID 36703008

Authors

Alejandro Lillo

Joan Serrano-Marin

Jaume Lillo

Iu Raich

Gemma Navarro

Rafael Franco

Affiliations

Soon will be listed here.

Abstract

Most neurodegenerative disorders, including the two most common, Alzheimer's disease (AD) and Parkinson's disease (AD), course with activation of microglia, the resident innate immune cells of the central nervous system. A adenosine receptor (AR) agonists have been proposed to be neuroprotective by regulating the phenotype of activated microglia. RNAseq was performed using samples isolated from lipopolysaccharide/interferon-γ activated microglia treated with 2-Cl-IB-MECA, a selective AR agonist. The results showed that the number of negatively regulated genes in the presence of 2-Cl-IB-MECA was greater than the number of positively regulated genes. Gene ontology enrichment analysis showed regulation of genes participating in several cell processes, including those involved in immune-related events. Analysis of known and predicted protein-protein interactions showed that Smad3 and Sp1 are transcription factors whose genes are regulated by AR activation. Under the conditions of cell activation and agonist treatment regimen, 2-Cl-IB-MECA did not lead to any tendency to favor the expression of genes related to neuroprotective microglia (M2).

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