Biocompatibility of Veratric Acid-Encapsulated Chitosan/Methylcellulose Hydrogel: Biological Characterization, Osteogenic Efficiency with In Silico Molecular Modeling

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2023 Jan 26

PMID 36701091

Authors

Kaliannan Durairaj

Balamuralikrishnan Balasubramanian

Vijaya Anand Arumugam

Murugesh Easwaran

Sungkwon Park

Utthapon Issara

Karthika Pushparaj

Naif Abdullah Al-Dhabi

Mariadhas Valan Arasu

Wen-Chao Liu

Amin Mousavi Khaneghah

Affiliations

Soon will be listed here.

Abstract

The limitations of graft material, and surgical sites for autografts in bone defects treatment have become a significant challenge in bone tissue engineering. Phytocompounds markedly affect bone metabolism by activating the osteogenic signaling pathways. The present study investigated the biocompatibility of the bio-composite thermo-responsive hydrogels consisting of chitosan (CS), and methylcellulose (MC) encapsulated with veratric acid (VA) as a restorative agent for bone defect treatment. The spectroscopy analyses confirmed the formation of CS/MC hydrogels and VA encapsulated CS/MC hydrogels (CS/MC-VA). Molecular analysis of the CS-specific MC decamer unit with VA complex exhibited a stable integration in the system. Further, Runx2 (runt-related transcription factor 2) was found in the docking mechanism with VA, indicating a high binding affinity towards the functional site of the Runx2 protein. The formulated CS/MC-VA hydrogels exhibited biocompatibility with the mouse mesenchymal stem cells, while VA promoted osteogenic differentiation in the stem cells, which was verified by calcium phosphate deposition through the von Kossa staining. The study results suggest that CS/MC-VA could be a potential therapeutic alternative source for bone regeneration.

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