Label-free Full-field Doppler Phase Microscopy Based on Optical Computation

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2023 Jan 26

PMID 36698679

Authors

Yuwei Liu

Shupei Yu

Yuanwei Zhang

Xuan Liu

Affiliations

Soon will be listed here.

Abstract

The capability to image subtle mechanical motion at cellular and sub-cellular scales can be used to study how extracellular particles interact with cultured cells and, more generally, how cells interact with their environment. However, current technologies need to provide sufficient spatial resolution, temporal resolution, and motion sensitivity to image cellular and sub-cellular motion in the en face plane. To address this unmet need, we investigate a full-field Doppler phase microscopy (FF-DPM) technology based on an innovative optical computation strategy that enables depth-resolved imaging and phase quantification. In this study, we validated the motion tracking (displacements and velocities) capability of FF-DPM by imaging samples actuated by a piezo transducer (PZT). We demonstrated FF-DPM imaging of magnetic particles under different conditions with different motion characteristics. Our results show that free particles (suspended in a cell culture medium) had a significantly larger magnitude of motion than particles adhered to a cell. The key innovation of this study is the use of an optical computation strategy to perform depth-resolved phase quantification and Doppler measurement. The FF-DPM will have a significant impact, as it provides a unique capability to quantitatively measure subtle motion for models based on cultured cells.

Citing Articles

Far-red BODIPY-based oxime esters: photo-uncaging and drug delivery.

Wan Z, Yu S, Wang Q, Sambath K, Harty R, Liu X J Mater Chem B. 2023; 11(41):9889-9893.

PMID: 37850246 PMC: 10750304. DOI: 10.1039/d3tb01867a.

References

Popescu G, DeFlores L, Vaughan J, Badizadegan K, Iwai H, Dasari R . Fourier phase microscopy for investigation of biological structures and dynamics. Opt Lett. 2004; 29(21):2503-5. DOI: 10.1364/ol.29.002503. View

Hillmann D, Bonin T, Luhrs C, Franke G, Hagen-Eggert M, Koch P . Common approach for compensation of axial motion artifacts in swept-source OCT and dispersion in Fourier-domain OCT. Opt Express. 2012; 20(6):6761-76. DOI: 10.1364/OE.20.006761. View

Yazdanfar S, Yang C, Sarunic M, Izatt J . Frequency estimation precision in Doppler optical coherence tomography using the Cramer-Rao lower bound. Opt Express. 2009; 13(2):410-6. DOI: 10.1364/opex.13.000410. View

Huang D, Swanson E, Lin C, Schuman J, Stinson W, Chang W . Optical coherence tomography. Science. 1991; 254(5035):1178-81. PMC: 4638169. DOI: 10.1126/science.1957169. View

Hendargo H, McNabb R, Dhalla A, Shepherd N, Izatt J . Doppler velocity detection limitations in spectrometer-based versus swept-source optical coherence tomography. Biomed Opt Express. 2011; 2(8):2175-88. PMC: 3149517. DOI: 10.1364/BOE.2.002175. View

Liu X, Wan Z, Zhang Y, Liu Y . Optically computed phase microscopy for quantitative dynamic imaging of label-free cells and nanoparticles. Biomed Opt Express. 2022; 13(1):514-524. PMC: 8803025. DOI: 10.1364/BOE.449034. View

Kashani A, Chen C, Gahm J, Zheng F, Richter G, Rosenfeld P . Optical coherence tomography angiography: A comprehensive review of current methods and clinical applications. Prog Retin Eye Res. 2017; 60:66-100. PMC: 5600872. DOI: 10.1016/j.preteyeres.2017.07.002. View

Su T, Xue L, Ozcan A . High-throughput lensfree 3D tracking of human sperms reveals rare statistics of helical trajectories. Proc Natl Acad Sci U S A. 2012; 109(40):16018-22. PMC: 3479566. DOI: 10.1073/pnas.1212506109. View

Dubois A, Selb J, Vabre L, Boccara A . Phase measurements with wide-aperture interferometers. Appl Opt. 2008; 39(14):2326-31. DOI: 10.1364/ao.39.002326. View

10.

Bonin T, Franke G, Hagen-Eggert M, Koch P, Huttmann G . In vivo Fourier-domain full-field OCT of the human retina with 1.5 million A-lines/s. Opt Lett. 2010; 35(20):3432-4. DOI: 10.1364/OL.35.003432. View

11.

Dai C, Liu X, Zhang H, Puliafito C, Jiao S . Absolute retinal blood flow measurement with a dual-beam Doppler optical coherence tomography. Invest Ophthalmol Vis Sci. 2013; 54(13):7998-8003. PMC: 3858018. DOI: 10.1167/iovs.13-12318. View

12.

Klein T, Huber R . High-speed OCT light sources and systems [Invited]. Biomed Opt Express. 2017; 8(2):828-859. PMC: 5330584. DOI: 10.1364/BOE.8.000828. View

13.

Vabre L, Dubois A, Boccara A . Thermal-light full-field optical coherence tomography. Opt Lett. 2007; 27(7):530-2. DOI: 10.1364/ol.27.000530. View

14.

Qiu Y, Wang Y, Xu Y, Chandra N, Haorah J, Hubbi B . Quantitative optical coherence elastography based on fiber-optic probe for in situ measurement of tissue mechanical properties. Biomed Opt Express. 2016; 7(2):688-700. PMC: 4771481. DOI: 10.1364/BOE.7.000688. View

15.

Ikeda T, Popescu G, Dasari R, Feld M . Hilbert phase microscopy for investigating fast dynamics in transparent systems. Opt Lett. 2005; 30(10):1165-7. DOI: 10.1364/ol.30.001165. View

16.

Wang Y, Kang Q, Zhang Y, Liu X . Optically computed optical coherence tomography for volumetric imaging. Opt Lett. 2020; 45(7):1675-1678. PMC: 7364404. DOI: 10.1364/OL.382045. View

17.

Liu X, Liu Y, Wan Z, Gunasekar A, Zhang Y . Quantitative dynamic cellular imaging based on 3D unwrapped optically computed phase microscopy. Appl Opt. 2022; 61(27):7999-8005. PMC: 9912344. DOI: 10.1364/AO.463843. View

18.

Zernike F . How I discovered phase contrast. Science. 1955; 121(3141):345-9. DOI: 10.1126/science.121.3141.345. View

19.

Langehanenberg P, Ivanova L, Bernhardt I, Ketelhut S, Vollmer A, Dirksen D . Automated three-dimensional tracking of living cells by digital holographic microscopy. J Biomed Opt. 2009; 14(1):014018. DOI: 10.1117/1.3080133. View

20.

Huang Y, Liu X, Kang J . Real-time 3D and 4D Fourier domain Doppler optical coherence tomography based on dual graphics processing units. Biomed Opt Express. 2012; 3(9):2162-74. PMC: 3447558. DOI: 10.1364/BOE.3.002162. View