Rapid PCR-lateral Flow Assay for the Onsite Detection of Atlantic White Shrimp

Overview

Journal Food Chem (Oxf)

Specialty Biochemistry

Date 2023 Jan 26

PMID 36698370

Authors

Samuel Kwawukume

Frank J Velez

David Williams

Leqi Cui

Prashant Singh

Affiliations

Soon will be listed here.

Abstract

The Atlantic white shrimp () is of great economic importance to the United States and risk being substituted with imported species due to a shortage in domestic production. To improve the current methods used for the identification of the Atlantic white shrimp species, we designed and validated a robust multiplex PCR-lateral flow assay for the onsite identification of . The standardized assay was validated using a miniaturized, low-cost PCR instrument with 68 shrimp, prawn, and fish samples, spread over fourteen seafood species. was simultaneously amplified by the multiplex assay to give three visual bands, which distinguished it from other species having either one or two bands on the dipstick. The standardized assay showed 100% inclusivity for target samples, 100% exclusivity for non-target samples and can be completed in less than two hours. The assay standardized in this study can be used for onsite testing of samples at processing facilities, restaurants, and wholesalers' facilities.

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