Application of Stichopus Hermanni Nanoparticle Gel in the Healing of Traumatic Ulcers

Overview

Journal Eur J Dent

Publisher Thieme

Specialty Dentistry

Date 2023 Jan 23

PMID 36690026

Authors

Rima Parwati Sari

Debora Intan Dewi Larashati

Clarissa Aldiana

Nafiah Nafiah

Dian Widya Damaiyanti

Atik Kurniawati

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this research was to investigate the use of nanoparticle gel on the ulcer healing process by observing blood vessels, fibroblasts, and Collagen type-I (COL-1) expression on the 4 and 7th days after trauma.

Materials And Methods: Gold sea cucumber () powder was processed by freeze-drying method, then by high-energy milling to form nanoparticle size, and then with CMC 2% to make hydrogel. Traumatic ulcers were formed by induction using a burner. Five groups of male Wistar rats, each consisting of six tails, were divided into a negative control group that was given a placebo, the positive control group was given 0.2% hyaluronic acid, and the treatment group was given gold sea cucumbers with concentrations of 0.135, 0.27, and 0.54% (SH1-SH2-SH3). Fibroblast and blood vessels were examined with hematoxylin-eosin on day 3 and 7, while COL-1 expression was examined with immunohistochemistry on day 7. The rats' mucosa was taken on the 3rd and 7th days after the traumatic ulcer was formed.

Statistical Analysis: The data were analyzed using a one-way analysis of variance followed by a post-hoc test with a less than 0.05.

Results: Nanoparticles gel freeze-drying of increased blood vessels on day 3. Angiogenesis continued to occur, which resulted in increased fibroblast and COL-1 expression on day 7.

Conclusions: The application of nanoparticle gel at 0.27% effectively increased the number of blood vessels, fibroblasts, and COL-1 expression in healing traumatic ulcers.

Citing Articles

TNF-α Expression and Plasma Cell and Macrophage Numbers in Traumatic Ulcers in a Rat Model Treated by Topical or Systemic Probiotic .

Savitri I, Suprarahardiani S, Ulinnuha L, Prahasanti C, Wijaksana I, Ridwan R Int J Med Sci. 2025; 22(2):318-327.

PMID: 39781533 PMC: 11704693. DOI: 10.7150/ijms.99156.

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