Polymorphisms in and Genes and Their Association with Feed Conversion Ratio in Hu Sheep

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2023 Jan 23

PMID 36686193

Authors

Changchun Lin

Weimin Wang

Deyin Zhang

Kai Huang

Xiaolong Li

Yukun Zhang

Yuan Zhao

Jianghui Wang

Bubo Zhou

Jiangbo Cheng

Dan Xu

Wenxin Li

Liming Zhao

Zongwu Ma

Xiaobin Yang

Yongliang Huang

Panpan Cui

Jia Liu

Xiwen Zeng

Rui Zhai

Landi Sun

Xiuxiu Weng

Weiwei Wu

Xiaoxue Zhang

Wenxin Zheng

Affiliations

Soon will be listed here.

Abstract

In animal husbandry, feed efficiency is a crucial economic trait. In this study, the general linear model was used to perform association analysis for various genotypes and feed conversion ratio (FCR)-related traits. Reverse transcription-quantitative PCR (RT-qPCR) was used to detect the expression of and mRNA levels in 10 tissues from 6 sheep. The results showed that SNPs in the NC_040257.1:c.625 T > C and NC_040261.1:g.9905 T > C were analyzed whether they were associated to feed efficiency parameters in Hu sheep (body weight, feed intake, average daily growth, and feed conversion ratio). NC_040257.1:c.625 T > C was shown to be significantly associated with body weight at 80, 100, and 120 days as well as feed conversion ratio ( < 0.05), whereas NC_040261.1:g.9905 T > C was found to be significantly associated with average daily weight gain from 80-140 days (ADG80-140) and FCR ( < 0.05). In Hu sheep, the CC genotypes of SHISA3 and RFC3 were the most common genotypes related to feed efficiency traits. Furthermore, the feed conversion ratio of the combined genotypes TT -CC , TT -CT , TT -TT , CT -CC and CT -CT was significantly better than the FCR of CC -TT . RT-qPCR results showed that the expression levels of were lower in the lung than in spleen, kidney, muscle and lymph ( < 0.05), and RFC3 was the lung had a highly significant higher expression level than the heart, liver, spleen, and muscle ( < 0.01). In conclusion, and polymorphisms can be used as genetic markers for improving feed conversion efficiency in Hu sheep.

Citing Articles

Effect of Feeding Level on Growth and Slaughter Performance, and Allometric Growth of Tissues and Organs in Female Growing Saanen Dairy Goats.

Huang J, Jiao S, Fu Y, Zhao W, Diao Q, Ma T Animals (Basel). 2024; 14(5).

PMID: 38473114 PMC: 10931386. DOI: 10.3390/ani14050730.

References

Zhao L, Li F, Liu T, Yuan L, Zhang X, Zhang D . Ovine ELOVL5 and FASN genes polymorphisms and their correlations with sheep tail fat deposition. Gene. 2021; 807:145954. DOI: 10.1016/j.gene.2021.145954. View

Pei J, Grishin N . Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell Signal. 2011; 24(3):758-69. PMC: 3295595. DOI: 10.1016/j.cellsig.2011.11.011. View

Onteru S, Gorbach D, Young J, Garrick D, Dekkers J, Rothschild M . Whole Genome Association Studies of Residual Feed Intake and Related Traits in the Pig. PLoS One. 2013; 8(6):e61756. PMC: 3694077. DOI: 10.1371/journal.pone.0061756. View

Karnam A, Rambabu N, Das M, Bou-Jaoudeh M, Delignat S, Kasermann F . Therapeutic normal IgG intravenous immunoglobulin activates Wnt-β-catenin pathway in dendritic cells. Commun Biol. 2020; 3(1):96. PMC: 7055225. DOI: 10.1038/s42003-020-0825-4. View

Do D, Ostersen T, Strathe A, Mark T, Jensen J, Kadarmideen H . Genome-wide association and systems genetic analyses of residual feed intake, daily feed consumption, backfat and weight gain in pigs. BMC Genet. 2014; 15:27. PMC: 3929553. DOI: 10.1186/1471-2156-15-27. View

Abo-Ismail M, Vander Voort G, Squires J, Swanson K, Mandell I, Liao X . Single nucleotide polymorphisms for feed efficiency and performance in crossbred beef cattle. BMC Genet. 2014; 15:14. PMC: 3927660. DOI: 10.1186/1471-2156-15-14. View

Chen C, Chen H, Su K, Hong Q, Yan B, Chen C . Shisa3 is associated with prolonged survival through promoting β-catenin degradation in lung cancer. Am J Respir Crit Care Med. 2014; 190(4):433-44. DOI: 10.1164/rccm.201312-2256OC. View

Pillay J, Kamp V, van Hoffen E, Visser T, Tak T, Lammers J . A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1. J Clin Invest. 2011; 122(1):327-36. PMC: 3248287. DOI: 10.1172/JCI57990. View

Uhlmann F, Cai J, Flores-Rozas H, Dean F, Finkelstein J, ODonnell M . In vitro reconstitution of human replication factor C from its five subunits. Proc Natl Acad Sci U S A. 1996; 93(13):6521-6. PMC: 39056. DOI: 10.1073/pnas.93.13.6521. View

10.

Cantalapiedra-Hijar G, Abo-Ismail M, Carstens G, Guan L, Hegarty R, Kenny D . Review: Biological determinants of between-animal variation in feed efficiency of growing beef cattle. Animal. 2018; 12(s2):s321-s335. DOI: 10.1017/S1751731118001489. View

11.

Sarker P, Kapuscinski A, McKuin B, Fitzgerald D, Nash H, Greenwood C . Microalgae-blend tilapia feed eliminates fishmeal and fish oil, improves growth, and is cost viable. Sci Rep. 2020; 10(1):19328. PMC: 7665073. DOI: 10.1038/s41598-020-75289-x. View

12.

Fang L, Cai W, Liu S, Canela-Xandri O, Gao Y, Jiang J . Comprehensive analyses of 723 transcriptomes enhance genetic and biological interpretations for complex traits in cattle. Genome Res. 2020; 30(5):790-801. PMC: 7263193. DOI: 10.1101/gr.250704.119. View

13.

Dickins B, Nekrutenko A . High-resolution mapping of evolutionary trajectories in a phage. Genome Biol Evol. 2010; 1:294-307. PMC: 2817424. DOI: 10.1093/gbe/evp029. View

14.

Delgado B, Bach A, Guasch I, Gonzalez C, Elcoso G, Pryce J . Author Correction: Whole rumen metagenome sequencing allows classifying and predicting feed efficiency and intake levels in cattle. Sci Rep. 2020; 10(1):2875. PMC: 7016186. DOI: 10.1038/s41598-020-60003-8. View

15.

Kang M, Ryu E, Lee S, Park J, Ha N, Ra J . Regulation of PCNA cycling on replicating DNA by RFC and RFC-like complexes. Nat Commun. 2019; 10(1):2420. PMC: 6546911. DOI: 10.1038/s41467-019-10376-w. View

16.

Fu L, Jiang Y, Wang C, Mei M, Zhou Z, Jiang Y . A Genome-Wide Association Study on Feed Efficiency Related Traits in Landrace Pigs. Front Genet. 2020; 11:692. PMC: 7350416. DOI: 10.3389/fgene.2020.00692. View

17.

Li W, Liu R, Zheng M, Feng F, Liu D, Guo Y . New insights into the associations among feed efficiency, metabolizable efficiency traits and related QTL regions in broiler chickens. J Anim Sci Biotechnol. 2020; 11:65. PMC: 7318453. DOI: 10.1186/s40104-020-00469-8. View

18.

Yamamoto A, Nagano T, Takehara S, Hibi M, Aizawa S . Shisa promotes head formation through the inhibition of receptor protein maturation for the caudalizing factors, Wnt and FGF. Cell. 2005; 120(2):223-35. DOI: 10.1016/j.cell.2004.11.051. View

19.

Murakami K, Zhifeng H, Suzuki T, Kobayashi Y, Nakamura Y . The Shisa3 knockout mouse exhibits normal bone phenotype. J Bone Miner Metab. 2019; 37(6):967-975. DOI: 10.1007/s00774-019-01014-y. View

20.

Volden H . Effects of level of feeding and ruminally undegraded protein on ruminal bacterial protein synthesis, escape of dietary protein, intestinal amino acid profile, and performance of dairy cows. J Anim Sci. 1999; 77(7):1905-18. DOI: 10.2527/1999.7771905x. View