Construction of a Mineralized Collagen Nerve Conduit for Peripheral Nerve Injury Repair

Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2023 Jan 23

PMID 36683739

Authors

Guman Duan

Chengli Li

Xiaoqing Yan

Shuhui Yang

Shuo Wang

Xiaodan Sun

Lingyun Zhao

Tianxi Song

Yongwei Pan

Xiumei Wang

Affiliations

Soon will be listed here.

Abstract

A new nerve guidance conduits (NGCs) named MC@Col containing Type I collagen (Col) and mineralized collagen (MC) was developed, enhancing mechanical and degradation behavior. The physicochemical properties, the mechanical properties and degradation behavior were all evaluated. The adhesion and proliferation of Schwann cells (SCs) were observed. In the experiment, MC@Col NGC and other conduits including Col, chitosan (CST) and polycaprolactone (PCL) conduit were implanted to repair a 10-mm-long Sprague-Dawley rat's sciatic nerve defect. Histological analyses, morphological analyses, electrophysiological analyses and further gait analyses were all evaluated after implantation in 12 weeks. The strength and degradation performance of the MC@Col NGC were improved by the addition of MC in comparison with pure Col NGC. cytocompatibility evaluation revealed that the SCs had good viability, attachment and proliferation in the MC@Col. In results, the regenerative outcomes of MC@Col NGC were close to those by an autologous nerve graft in some respects, but superior to those by Col, CST and PCL conduits. The MC@Col NGC exhibited good mechanical performance as well as biocompatibility to bridge nerve gap and guide nerve regeneration, thus showing great promising potential as a new type of conduit in clinical applications.

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References

Lu J, Yan X, Sun X, Shen X, Yin H, Wang C . Synergistic effects of dual-presenting VEGF- and BDNF-mimetic peptide epitopes from self-assembling peptide hydrogels on peripheral nerve regeneration. Nanoscale. 2019; 11(42):19943-19958. DOI: 10.1039/c9nr04521j. View

Sutherland D, Pujic Z, Goodhill G . Calcium signaling in axon guidance. Trends Neurosci. 2014; 37(8):424-32. DOI: 10.1016/j.tins.2014.05.008. View

Seo K, Terumitsu M, Inada Y, Nakamura T, Shigeno K, Tanaka Y . Prognosis After Surgical Treatment of Trigeminal Neuropathy with a PGA-c Tube: Report of 10 Cases. Pain Med. 2016; 17(12):2360-2368. DOI: 10.1093/pm/pnw088. View

Johnson E, Soucacos P . Nerve repair: experimental and clinical evaluation of biodegradable artificial nerve guides. Injury. 2008; 39 Suppl 3:S30-6. DOI: 10.1016/j.injury.2008.05.018. View

Liang C, Luo Y, Yang G, Xia D, Liu L, Zhang X . Graphene Oxide Hybridized nHAC/PLGA Scaffolds Facilitate the Proliferation of MC3T3-E1 Cells. Nanoscale Res Lett. 2018; 13(1):15. PMC: 5764901. DOI: 10.1186/s11671-018-2432-6. View

Henley J, Poo M . Guiding neuronal growth cones using Ca2+ signals. Trends Cell Biol. 2004; 14(6):320-30. PMC: 3115711. DOI: 10.1016/j.tcb.2004.04.006. View

Du J, Liu J, Yao S, Mao H, Peng J, Sun X . Prompt peripheral nerve regeneration induced by a hierarchically aligned fibrin nanofiber hydrogel. Acta Biomater. 2017; 55:296-309. DOI: 10.1016/j.actbio.2017.04.010. View

Nawrotek K, Tylman M, Rudnicka K, Gatkowska J, Balcerzak J . Tubular electrodeposition of chitosan-carbon nanotube implants enriched with calcium ions. J Mech Behav Biomed Mater. 2016; 60:256-266. DOI: 10.1016/j.jmbbm.2016.02.012. View

Meyer C, Stenberg L, Gonzalez-Perez F, Wrobel S, Ronchi G, Udina E . Chitosan-film enhanced chitosan nerve guides for long-distance regeneration of peripheral nerves. Biomaterials. 2015; 76:33-51. DOI: 10.1016/j.biomaterials.2015.10.040. View

10.

Zhuang J, Lin J, Li J, Wang H, Cheng K, Weng W . Electrochemical deposition of mineralized BSA/collagen coating. Mater Sci Eng C Mater Biol Appl. 2016; 66:66-76. DOI: 10.1016/j.msec.2016.04.088. View

11.

Chen L, Tang C, Tsui C, Chen D . Mechanical properties and in vitro evaluation of bioactivity and degradation of dexamethasone-releasing poly-D-L-lactide/nano-hydroxyapatite composite scaffolds. J Mech Behav Biomed Mater. 2013; 22:41-50. DOI: 10.1016/j.jmbbm.2013.03.008. View

12.

Ding C, Du J, Cao Y, Yue C, Cheng B . Effects of the aspect ratio of multi-walled carbon nanotubes on the structure and properties of regenerated collagen fibers. Int J Biol Macromol. 2018; 126:595-602. DOI: 10.1016/j.ijbiomac.2018.12.144. View

13.

Chang M, Tanaka J . FT-IR study for hydroxyapatite/collagen nanocomposite cross-linked by glutaraldehyde. Biomaterials. 2002; 23(24):4811-8. DOI: 10.1016/s0142-9612(02)00232-6. View

14.

Quan Q, Meng H, Chang B, Liu G, Cheng X, Tang H . Aligned fibers enhance nerve guide conduits when bridging peripheral nerve defects focused on early repair stage. Neural Regen Res. 2019; 14(5):903-912. PMC: 6375037. DOI: 10.4103/1673-5374.249239. View

15.

Quan Q, Meng H, Chang B, Hong L, Li R, Liu G . Novel 3-D helix-flexible nerve guide conduits repair nerve defects. Biomaterials. 2019; 207:49-60. DOI: 10.1016/j.biomaterials.2019.03.040. View

16.

Chomiak T, Hu B . What is the optimal value of the g-ratio for myelinated fibers in the rat CNS? A theoretical approach. PLoS One. 2009; 4(11):e7754. PMC: 2771903. DOI: 10.1371/journal.pone.0007754. View

17.

Ahmed M, Vairamuthu S, Shafiuzama M, Basha S, Jayakumar R . Microwave irradiated collagen tubes as a better matrix for peripheral nerve regeneration. Brain Res. 2005; 1046(1-2):55-67. DOI: 10.1016/j.brainres.2005.03.022. View

18.

Wang J, Pin Yeo K, Chun Y, Tan T, Tan N, Angeli V . Fish scale-derived collagen patch promotes growth of blood and lymphatic vessels in vivo. Acta Biomater. 2017; 63:246-260. DOI: 10.1016/j.actbio.2017.09.001. View

19.

Connor J . Digital imaging of free calcium changes and of spatial gradients in growing processes in single, mammalian central nervous system cells. Proc Natl Acad Sci U S A. 1986; 83(16):6179-83. PMC: 386463. DOI: 10.1073/pnas.83.16.6179. View

20.

Bain J, Mackinnon S, Hunter D . Functional evaluation of complete sciatic, peroneal, and posterior tibial nerve lesions in the rat. Plast Reconstr Surg. 1989; 83(1):129-38. DOI: 10.1097/00006534-198901000-00024. View