Impact of Swine and Cattle Manure Treatment on the Microbial Composition and Resistome of Soil and Drainage Water

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Jan 21

PMID 36677309

Authors

Phil Colgan

Elizabeth L Rieke

Khurram Nadeem

Thomas B Moorman

Michelle L Soupir

Adina Howe

Nicole Ricker

Affiliations

Soon will be listed here.

Abstract

Evaluating potential environmental and clinical impacts of industrial antibiotic use is critical in mitigating the spread of antimicrobial resistance. Using soil columns to simulate field application of swine or cattle manure and subsequent rain events, and a targeted qPCR-based approach, we tracked resistance genes from source manures and identified important differences in antimicrobial resistance gene transport and enrichment over time in the soil and water of artificially drained cropland. The source manures had distinct microbial community and resistance gene profiles, and these differences were also reflected in the soil columns after manure application. Antibiotic resistance genes (ARGs) were only significantly enriched in effluent samples following the first rain event (day 11) for both soil types compared to the control columns, illustrating the high background level of resistance present in the control soils chosen. For swine, the genes , , , , and persisted in the soil columns, whereas , and persisted in effluent samples. Conversely, for cattle manure and persisted in both soil and effluent. The distinct temporal dynamics of ARG distribution between soil and effluent water for each manure type can be used to inform potential mitigation strategies in the future.

Citing Articles

Dynamic interaction of antibiotic resistance between plant microbiome and organic fertilizers: sources, dissemination, and health risks.

Ullah H, Hassan S, Yang Q, Salama E, Liu P, Li X World J Microbiol Biotechnol. 2024; 41(1):4.

PMID: 39690351 DOI: 10.1007/s11274-024-04214-5.

Special Issue "Multidrug-Resistant Bacteria in the Environment, Their Resistance and Transfer Mechanisms".

Grohmann E Microorganisms. 2023; 11(4).

PMID: 37110404 PMC: 10141088. DOI: 10.3390/microorganisms11040981.

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