An Immunomodulatory Polysaccharide-Protein Complex Isolated from the Polypore Fungus

Overview

Journal J Fungi (Basel)

Date 2023 Jan 21

PMID 36675908

Authors

Bryan C C Lim

Mehreen Zeb

Wai-Ming Li

John Z Tang

Christian Heiss

Linda E Tackaberry

Hugues B Massicotte

Keith N Egger

Kerry Reimer

Parastoo Azadi

Chow H Lee

Affiliations

Soon will be listed here.

Abstract

Many wild edible polypore mushrooms have medicinal value. In this study, we investigate the potential medicinal properties of the wild polypore mushroom collected from north-central British Columbia, Canada. Water extract from was found to exhibit potent immunomodulatory activity. The extract was purified using DEAE-Sephadex anion-exchange chromatography as well as Sephacryl S-500 and HPLC BioSEC5 size-exclusion chromatography, to yield a novel polysaccharide-protein complex (IMPP-Rb).IMPP-Rb has a peak maxima molecular weight (M) of 950 kDa. GC-MS analyses showed that IMPP-Rb is composed predominantly of glucose (49.2%), galactose (11.3%), mannose (10.8%), rhamnose (9.6%), and galacturonic acid (8.2%), with smaller amounts of xylose (5.2%), fucose (2.8%), N-acetyl glucosamine (1.8%), and arabinose (1.2%). IMPP-Rb has multiple linkages, with 4-Glc, 4-Man, 6-Man, 3,4-Man, 4-Xyl, and 2-Rha being the most prominent. IMPP-Rb is capable of inducing many cytokines in vitro and the protein component is indispensable for its immunomodulatory activity. IMPP-Rb has potential application as an immuno-stimulatory agent with pharmaceutical value.

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