Preparation and In Vitro Characterization of Magnetic CS/PVA/HA/pSPIONs Scaffolds for Magnetic Hyperthermia and Bone Regeneration

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Jan 21

PMID 36674644

Authors

Francisco J T M Tavares

Paula I P Soares

Jorge Carvalho Silva

Joao Paulo Borges

Affiliations

Soon will be listed here.

Abstract

Conventional bone cancer treatment often results in unwanted side effects, critical-sized bone defects, and inefficient cancer-cell targeting. Therefore, new approaches are necessary to better address bone cancer treatment and patient's recovery. One solution may reside in the combination of bone regeneration scaffolds with magnetic hyperthermia. By incorporating pristine superparamagnetic iron oxide nanoparticles (pSPIONs) into additively manufactured scaffolds we created magnetic structures for magnetic hyperthermia and bone regeneration. For this, hydroxyapatite (HA) particles were integrated in a polymeric matrix composed of chitosan (CS) and poly (vinyl alcohol) (PVA). Once optimized, pSPIONs were added to the CS/PVA/HA paste at three different concentrations (1.92, 3.77, and 5.54 wt.%), and subsequently additively manufactured to form a scaffold. Results indicate that scaffolds containing 3.77 and 5.54 wt.% of pSPIONs, attained temperature increases of 6.6 and 7.5 °C in magnetic hyperthermia testing, respectively. In vitro studies using human osteosarcoma Saos-2 cells indicated that pSPIONs incorporation significantly stimulated cell adhesion, proliferation and alkaline phosphatase (ALP) expression when compared to CS/PVA/HA scaffolds. Thus, these results support that CS/PVA/HA/pSPIONs scaffolds with pSPIONs concentrations above or equal to 3.77 wt.% have the potential to be used for magnetic hyperthermia and bone regeneration.

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References

Paulino A . Late effects of radiotherapy for pediatric extremity sarcomas. Int J Radiat Oncol Biol Phys. 2004; 60(1):265-74. DOI: 10.1016/j.ijrobp.2004.02.001. View

Zhang Y, Yang J, Zhao N, Wang C, Kamar S, Zhou Y . Progress in the chemotherapeutic treatment of osteosarcoma. Oncol Lett. 2018; 16(5):6228-6237. PMC: 6202490. DOI: 10.3892/ol.2018.9434. View

Sharma A, Brand D, Fairbank J, Ye H, Lavy C, Czernuszka J . A self-organising biomimetic collagen/nano-hydroxyapatite-glycosaminoglycan scaffold for spinal fusion. J Mater Sci. 2018; 52(21):12574-12592. PMC: 6029624. DOI: 10.1007/s10853-017-1229-9. View

Chan Y, Marshall W . Scaling properties of cell and organelle size. Organogenesis. 2010; 6(2):88-96. PMC: 2901812. DOI: 10.4161/org.6.2.11464. View

Kharazmi A, Faraji N, Mat Hussin R, Saion E, Yunus W, Behzad K . Structural, optical, opto-thermal and thermal properties of ZnS-PVA nanofluids synthesized through a radiolytic approach. Beilstein J Nanotechnol. 2015; 6:529-36. PMC: 4362026. DOI: 10.3762/bjnano.6.55. View

Matsumine A, Takegami K, Asanuma K, Matsubara T, Nakamura T, Uchida A . A novel hyperthermia treatment for bone metastases using magnetic materials. Int J Clin Oncol. 2011; 16(2):101-8. DOI: 10.1007/s10147-011-0217-3. View

Pahlevanzadeh F, Emadi R, Valiani A, Kharaziha M, Poursamar S, Bakhsheshi-Rad H . Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends. Materials (Basel). 2020; 13(11). PMC: 7321644. DOI: 10.3390/ma13112663. View

Pineda-Castillo S, Bernal-Ballen A, Bernal-Lopez C, Segura-Puello H, Nieto-Mosquera D, Villamil-Ballesteros A . Synthesis and Characterization of Poly(Vinyl Alcohol)-Chitosan-Hydroxyapatite Scaffolds: A Promising Alternative for Bone Tissue Regeneration. Molecules. 2018; 23(10). PMC: 6222900. DOI: 10.3390/molecules23102414. View

Tamburaci S, Tihminlioglu F . Development of Si doped nano hydroxyapatite reinforced bilayer chitosan nanocomposite barrier membranes for guided bone regeneration. Mater Sci Eng C Mater Biol Appl. 2021; 128:112298. DOI: 10.1016/j.msec.2021.112298. View

10.

Zadpoor A, Malda J . Additive Manufacturing of Biomaterials, Tissues, and Organs. Ann Biomed Eng. 2016; 45(1):1-11. DOI: 10.1007/s10439-016-1719-y. View

11.

Chopin-Doroteo M, Mandujano-Tinoco E, Krotzsch E . Tailoring of the rheological properties of bioinks to improve bioprinting and bioassembly for tissue replacement. Biochim Biophys Acta Gen Subj. 2020; 1865(2):129782. DOI: 10.1016/j.bbagen.2020.129782. View

12.

Molyneaux K, Wnek M, Craig S, Vincent J, Rucker I, Wnek G . Physically-cross-linked poly(vinyl alcohol) cell culture plate coatings facilitate preservation of cell-cell interactions, spheroid formation, and stemness. J Biomed Mater Res B Appl Biomater. 2021; 109(11):1744-1753. DOI: 10.1002/jbm.b.34832. View

13.

Kang H, Hu S, Cho M, Hong S, Choi Y, Choi H . Theranostic Nanosystems for Targeted Cancer Therapy. Nano Today. 2019; 23:59-72. PMC: 6559746. DOI: 10.1016/j.nantod.2018.11.001. View

14.

Schneider C, Rasband W, Eliceiri K . NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012; 9(7):671-5. PMC: 5554542. DOI: 10.1038/nmeth.2089. View

15.

Chittajallu S, Richhariya A, Ming Tse K, Chinthapenta V . A Review on Damage and Rupture Modelling for Soft Tissues. Bioengineering (Basel). 2022; 9(1). PMC: 8773318. DOI: 10.3390/bioengineering9010026. View

16.

Alhosseini S, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A . Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int J Nanomedicine. 2012; 7:25-34. PMC: 3260948. DOI: 10.2147/IJN.S25376. View

17.

Toledo S, Oliveira I, Okamoto O, Zago M, Alves M, Filho R . Bone deposition, bone resorption, and osteosarcoma. J Orthop Res. 2010; 28(9):1142-8. DOI: 10.1002/jor.21120. View

18.

Liu F, Li W, Liu H, Yuan T, Yang Y, Zhou W . Preparation of 3D Printed Chitosan/Polyvinyl Alcohol Double Network Hydrogel Scaffolds. Macromol Biosci. 2021; 21(4):e2000398. DOI: 10.1002/mabi.202000398. View

19.

Soares P, Lochte F, Echeverria C, Pereira L, Coutinho J, Ferreira I . Thermal and magnetic properties of iron oxide colloids: influence of surfactants. Nanotechnology. 2015; 26(42):425704. DOI: 10.1088/0957-4484/26/42/425704. View

20.

Bassi G, Panseri S, Dozio S, Sandri M, Campodoni E, Dapporto M . Scaffold-based 3D cellular models mimicking the heterogeneity of osteosarcoma stem cell niche. Sci Rep. 2020; 10(1):22294. PMC: 7749131. DOI: 10.1038/s41598-020-79448-y. View