Titanium Dioxide Nanoparticles Altered the LncRNA Expression Profile in Human Lung Cells

Overview

Journal Int J Environ Res Public Health

Publisher MDPI

Specialties Environmental Health
Public Health

Date 2023 Jan 21

PMID 36673815

Authors

Ying Ma

Jiaqi Shi

Yi Zhang

Zhangjian Chen

Guang Jia

Affiliations

Soon will be listed here.

Abstract

Respiration is considered to be the main occupational or environmental exposure pathway of titanium dioxide nanoparticles (TiO NPs), and the lung is considered to be the target organ of respiratory exposure; however, the mechanism of respiratory toxicity is not fully understood. In this study, the effect of TiO NPs on the expression profile of long non-coding RNA (lncRNA) in bronchial epithelial cells (BEAS-2B) was investigated to understand their potential toxic mechanism. BEAS-2B cells were treated with 100 μg/mL TiO NPs for 48 h, then RNA sequencing was performed to screen the differential lncRNAs compared with the control group, and the enrichment pathways of the differentially expressed lncRNAs were further analyzed using the Kyoto Encyclopedia of Genes and Genomes (KEGG). The results identified a total of 45,769 lncRNAs, and 277 different lncRNAs were screened. KEGG pathway analysis showed that the targeted mRNAs of these different lncRNAs were enriched in the pyrimidine metabolism pathway. This work demonstrates that TiO NPs could alter the lncRNA expression profile in BEAS-2B cells, and epigenetics may play a role in the mechanism of respiratory toxicity induced by TiO NPs.

Citing Articles

Interaction of TiO nanoparticles with lung fluid proteins and the resulting macrophage inflammatory response.

Poulsen K, Albright M, Niemuth N, Tighe R, Payne C Environ Sci Nano. 2023; 10(9):2427-2436.

PMID: 38009084 PMC: 10669912. DOI: 10.1039/d3en00179b.

References

Biola-Clier M, Beal D, Caillat S, Libert S, Armand L, Herlin-Boime N . Comparison of the DNA damage response in BEAS-2B and A549 cells exposed to titanium dioxide nanoparticles. Mutagenesis. 2016; 32(1):161-172. DOI: 10.1093/mutage/gew055. View

Bai W, Chen Y, Gao A . Cross talk between poly(ADP-ribose) polymerase 1 methylation and oxidative stress involved in the toxic effect of anatase titanium dioxide nanoparticles. Int J Nanomedicine. 2015; 10:5561-9. PMC: 4562766. DOI: 10.2147/IJN.S88059. View

Gottschalk F, Nowack B . The release of engineered nanomaterials to the environment. J Environ Monit. 2011; 13(5):1145-55. DOI: 10.1039/c0em00547a. View

Al-Kattan A, Wichser A, Vonbank R, Brunner S, Ulrich A, Zuin S . Characterization of materials released into water from paint containing nano-SiO2. Chemosphere. 2014; 119:1314-1321. DOI: 10.1016/j.chemosphere.2014.02.005. View

Kanduri C . Long noncoding RNAs: Lessons from genomic imprinting. Biochim Biophys Acta. 2015; 1859(1):102-11. DOI: 10.1016/j.bbagrm.2015.05.006. View

Peschansky V, Wahlestedt C . Non-coding RNAs as direct and indirect modulators of epigenetic regulation. Epigenetics. 2014; 9(1):3-12. PMC: 3928183. DOI: 10.4161/epi.27473. View

Lv J, Qiu M, Xia W, Liu C, Xu Y, Wang J . High expression of long non-coding RNA SBF2-AS1 promotes proliferation in non-small cell lung cancer. J Exp Clin Cancer Res. 2016; 35:75. PMC: 4859961. DOI: 10.1186/s13046-016-0352-9. View

Garavito M, Narvaez-Ortiz H, Zimmermann B . Pyrimidine Metabolism: Dynamic and Versatile Pathways in Pathogens and Cellular Development. J Genet Genomics. 2015; 42(5):195-205. DOI: 10.1016/j.jgg.2015.04.004. View

Law P, Holland M . DNA methylation at the crossroads of gene and environment interactions. Essays Biochem. 2019; 63(6):717-726. PMC: 6923319. DOI: 10.1042/EBC20190031. View

10.

Jayaram D, Payne C . Food-Grade TiO Particles Generate Intracellular Superoxide and Alter Epigenetic Modifiers in Human Lung Cells. Chem Res Toxicol. 2020; 33(11):2872-2879. DOI: 10.1021/acs.chemrestox.0c00331. View

11.

Fatica A, Bozzoni I . Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet. 2013; 15(1):7-21. DOI: 10.1038/nrg3606. View

12.

Clemson C, McNeil J, Willard H, Lawrence J . XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure. J Cell Biol. 1996; 132(3):259-75. PMC: 2120729. DOI: 10.1083/jcb.132.3.259. View

13.

Zhang J, Shi J, Han S, Zheng P, Chen Z, Jia G . Titanium dioxide nanoparticles induced reactive oxygen species (ROS) related changes of metabolomics signatures in human normal bronchial epithelial (BEAS-2B) cells. Toxicol Appl Pharmacol. 2022; 444:116020. DOI: 10.1016/j.taap.2022.116020. View

14.

Li B, Huang N, Wei S, Xv J, Meng Q, Aschner M . lncRNA TUG1 as a ceRNA promotes PM exposure-induced airway hyper-reactivity. J Hazard Mater. 2021; 416:125878. PMC: 8432742. DOI: 10.1016/j.jhazmat.2021.125878. View

15.

Peng F, Dai J, Qian Q, Cao X, Wang L, Zhu M . Serum metabolic profiling of coal worker's pneumoconiosis using untargeted lipidomics. Environ Sci Pollut Res Int. 2022; 29(56):85444-85453. DOI: 10.1007/s11356-022-21905-4. View

16.

Moreira L, Costa C, Pires J, Teixeira J, Fraga S . How can exposure to engineered nanomaterials influence our epigenetic code? A review of the mechanisms and molecular targets. Mutat Res Rev Mutat Res. 2021; 788:108385. DOI: 10.1016/j.mrrev.2021.108385. View

17.

Zhen S, Qian Q, Jia G, Zhang J, Chen C, Wei Y . A panel study for cardiopulmonary effects produced by occupational exposure to inhalable titanium dioxide. J Occup Environ Med. 2012; 54(11):1389-94. DOI: 10.1097/JOM.0b013e3182611a49. View

18.

Jiang T, Wang H, Liu L, Song H, Zhang Y, Wang J . CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer. Mol Cancer. 2021; 20(1):167. PMC: 8684286. DOI: 10.1186/s12943-021-01474-9. View

19.

Lim J, Kim S, Kim B, Park S . Knockdown of CARD14 Inhibits Cell Proliferation and Migration in Breast Cancer Cells. Anticancer Res. 2020; 40(4):1953-1962. DOI: 10.21873/anticanres.14150. View

20.

Yamano S, Takeda T, Goto Y, Hirai S, Furukawa Y, Kikuchi Y . No evidence for carcinogenicity of titanium dioxide nanoparticles in 26-week inhalation study in rasH2 mouse model. Sci Rep. 2022; 12(1):14969. PMC: 9440215. DOI: 10.1038/s41598-022-19139-y. View