Volatile Profiling and Transcriptome Sequencing Provide Insights into the Biosynthesis of α-Pinene and β-Pinene in Hance Leaves

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Jan 21

PMID 36672904

Authors

Yongquan Li

Yanfang Gao

Lin Deng

Huiming Lian

Wei Guo

Wei Wu

Bine Xue

Baobin Li

Yuzhen Su

Hui Zhang

Affiliations

Soon will be listed here.

Abstract

Hance is a pinene-rich deciduous plant species in the Altingiaceae family that is used as a medicinal plant in China. However, the regulatory mechanisms underlying α-pinene and β-pinene biosynthesis in leaves remain unknown. Here, a joint analysis of the volatile compounds and transcriptomes of leaves was performed to comprehensively explore the terpene synthase (TPS) that may participate in α-pinene and β-pinene biosynthesis. Headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) jointly detected volatile leaves. Trees with high and low levels of both α-pinene and β-pinene were defined as the H group and L group, respectively. RNA sequencing data revealed that DXR (1-deoxy-D-xylulose-5-phosphate reductoisomerase), HDS [(E)-4-hydroxy-3-methylbut-2-eny-l-diphosphate synthase], and TPS may be the major regulators of monoterpenoid biosynthesis. We identified three TPSs (, and ), which are highly homologous to α-pinene and β-pinene synthases of other species in phylogenetic analysis. Four TPS genes (, , , ) may be critically involved in the biosynthesis and regulation of α-pinene and β-pinene in . Bioinformatic and transcriptomic results were verified using quantitative real-time PCR. We identified as candidate genes for α-pinene and β-pinene biosynthesis that significantly improve the yield of beneficial terpenoids.

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