Molecular, Physiological and Hematological Responses of Crossbred Dairy Cattle in a Tropical Savanna Climate

Overview

Journal Biology (Basel)

Publisher MDPI

Specialty Biology

Date 2023 Jan 21

PMID 36671719

Authors

Silpa Mullakkalparambil Velayudhan

Kerstin Brugemann

Shahin Alam

Tong Yin

Chinnasamy Devaraj

Veerasamy Sejian

Eva Schlecht

Sven Konig

Affiliations

Soon will be listed here.

Abstract

A comprehensive study was conducted to assess the effects of seasonal transition and temperature humidity index (THI) on the adaptive responses in crossbred dairy cows reared in a tropical savanna region. A total of 40 lactating dairy cattle reared by small-scale dairy farmers in Bengaluru, India, were selected for this study. The research period comprised the transitioning season of summer to monsoon, wherein all traits were recorded at two points, one representing late summer (June) and the other early monsoon (July). A set of extensive variables representing physiological responses (pulse rate, respiration rate, rectal temperature, skin surface temperature), hematological responses (hematological profile), production (test day milk yield, milk composition) and molecular patterns (PBMC mRNA relative expression of selective stress response genes) were assessed. A significant effect of seasonal transition was identified on respiration rate (RR), skin surface temperature, mean platelet volume (MPV), platelet distribution width (PDWc), test day milk yield and on milk composition variables (milk density, lactose, solids-not-fat (SNF) and salts). The THI had a significant effect on RR, skin surface temperature, platelet count (PLT), plateletcrit (PCT) and PDWc. Lastly, THI and/or seasonal transition significantly affected the relative PBMC mRNA expression of (), (), , (), () and () genes. The results from this study reveal environmental sensitivity of novel physiological traits and gene expressions to climatic stressors, highlighting their potential as THI-independent heat stress biomarkers.

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References

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

Mishra S . Behavioural, physiological, neuro-endocrine and molecular responses of cattle against heat stress: an updated review. Trop Anim Health Prod. 2021; 53(3):400. DOI: 10.1007/s11250-021-02790-4. View

Collier R, Collier J, Rhoads R, Baumgard L . Invited review: genes involved in the bovine heat stress response. J Dairy Sci. 2008; 91(2):445-54. DOI: 10.3168/jds.2007-0540. View

Sejian V, Bhatta R, Gaughan J, Dunshea F, Lacetera N . Review: Adaptation of animals to heat stress. Animal. 2018; 12(s2):s431-s444. DOI: 10.1017/S1751731118001945. View

Joudrey E, Lechniak D, Petrik J, King W . Expression of growth hormone and its transcription factor, Pit-1, in early bovine development. Mol Reprod Dev. 2003; 64(3):275-83. DOI: 10.1002/mrd.10237. View

Aleena J, Sejian V, Bagath M, Krishnan G, Beena V, Bhatta R . Resilience of three indigenous goat breeds to heat stress based on phenotypic traits and PBMC HSP70 expression. Int J Biometeorol. 2018; 62(11):1995-2005. DOI: 10.1007/s00484-018-1604-5. View

Jo J, Nejad J, Peng D, Kim H, Kim S, Lee H . Characterization of Short-Term Heat Stress in Holstein Dairy Cows Using Altered Indicators of Metabolomics, Blood Parameters, Milk MicroRNA-216 and Characteristics. Animals (Basel). 2021; 11(3). PMC: 8000480. DOI: 10.3390/ani11030722. View

Roland L, Drillich M, Iwersen M . Hematology as a diagnostic tool in bovine medicine. J Vet Diagn Invest. 2014; 26(5):592-8. DOI: 10.1177/1040638714546490. View

Collier R, Stiening C, Pollard B, Vanbaale M, Baumgard L, Gentry P . Use of gene expression microarrays for evaluating environmental stress tolerance at the cellular level in cattle. J Anim Sci. 2006; 84 Suppl:E1-13. DOI: 10.2527/2006.8413_supple1x. View

10.

George J, Snipes J, Lane V . Comparison of bovine hematology reference intervals from 1957 to 2006. Vet Clin Pathol. 2010; 39(2):138-48. DOI: 10.1111/j.1939-165X.2009.00208.x. View

11.

Zhou Y, Akers R, Jiang H . Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells. J Dairy Sci. 2007; 91(1):100-8. DOI: 10.3168/jds.2007-0509. View

12.

Martello L, Savastano Jr H, Silva S, Balieiro J . Alternative body sites for heat stress measurement in milking cows under tropical conditions and their relationship to the thermal discomfort of the animals. Int J Biometeorol. 2009; 54(6):647-52. DOI: 10.1007/s00484-009-0268-6. View

13.

Bai H, Ukita H, Kawahara M, Mitani T, Furukawa E, Yanagawa Y . Effect of summer heat stress on gene expression in bovine uterine endometrial tissues. Anim Sci J. 2020; 91(1):e13474. DOI: 10.1111/asj.13474. View

14.

Ferdous F, Scott T . A comparative examination of thrombocyte/platelet immunity. Immunol Lett. 2014; 163(1):32-9. DOI: 10.1016/j.imlet.2014.11.010. View

15.

Collier R, Dahl G, Vanbaale M . Major advances associated with environmental effects on dairy cattle. J Dairy Sci. 2006; 89(4):1244-53. DOI: 10.3168/jds.S0022-0302(06)72193-2. View

16.

Collier R, Baumgard L, Zimbelman R, Xiao Y . Heat stress: physiology of acclimation and adaptation. Anim Front. 2020; 9(1):12-19. PMC: 6951893. DOI: 10.1093/af/vfy031. View

17.

Alhussien M, Dang A . Potential roles of neutrophils in maintaining the health and productivity of dairy cows during various physiological and physiopathological conditions: a review. Immunol Res. 2019; 67(1):21-38. DOI: 10.1007/s12026-019-9064-5. View

18.

Yan G, Liu K, Hao Z, Shi Z, Li H . The effects of cow-related factors on rectal temperature, respiration rate, and temperature-humidity index thresholds for lactating cows exposed to heat stress. J Therm Biol. 2021; 100:103041. DOI: 10.1016/j.jtherbio.2021.103041. View

19.

Hull K, Harvey S . Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions. Int J Endocrinol. 2015; 2014:234014. PMC: 4279787. DOI: 10.1155/2014/234014. View

20.

Parmar P, Lopez-Villalobos N, Tobin J, Murphy E, McDonagh A, Crowley S . The Effect of Compositional Changes Due to Seasonal Variation on Milk Density and the Determination of Season-Based Density Conversion Factors for Use in the Dairy Industry. Foods. 2020; 9(8). PMC: 7466286. DOI: 10.3390/foods9081004. View