Overexpression of , an -like Gene of Bamboo, Causes Early Flowering and Abnormal Floral Organs in and Rice

Overview

Journal Acta Biochim Biophys Sin (Shanghai)

Specialties Biochemistry
Biophysics

Date 2023 Jan 17

PMID 36647724

Authors

Shinan Liu

Dan Hou

Naresh Vasupalli

Xinchun Lin

Affiliations

Soon will be listed here.

Abstract

Bamboo is a nontimber woody plant featuring a long vegetative stage and uncertain flowering time. Therefore, the genes belonging to flowering repressors might be essential in regulating the transition from the vegetative to reproductive stage in bamboo. The ( ) gene plays a pivotal role in floral transition and development. However, little is known about the bamboo homologues. In this study, is isolated by analysis of the . transcriptome database. Phylogenetic analysis shows that is closely related to (rice homolog). is ubiquitously expressed in various tissues, predominantly in vegetative tissues. To investigate the function of , is overexpressed in and rice under the influence of the 35S promoter. Overexpression of in causes early flowering and produces abnormal petals and sepals. Quantitative real-time PCR reveals that overexpression in produces an early flowering phenotype by downregulating and upregulating and produces abnormal floral organs by upregulating , and expressions. Simultaneously, overexpression of in rice alters the expressions of flowering-related genes such as , , and and promotes flowering under field conditions. In addition, PvSVP1 may be a nuclear protein which interacts with PvVRN1 and PvMADS56 on the yeast two-hybrid and BiFC systems. Our study suggests that may play a vital role in flowering time and development by interacting with PvVRN1 and PvMADS56 in the nucleus. Furthermore, this study paves the way toward understanding the complex flowering process of bamboo.

Citing Articles

Haplotype-resolved nonaploid genome provides insights into flowering in bamboos.

Wang Y, Guo C, Zhao L, Mao L, Hu X, Yang Y Hortic Res. 2024; 11(12):uhae250.

PMID: 39664687 PMC: 11630085. DOI: 10.1093/hr/uhae250.

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