Cloning and Expression Study of a High-affinity Nitrate Transporter Gene from

Overview

Journal Plant Signal Behav

Specialty Biology

Date 2023 Jan 16

PMID 36645908

Authors

Guoliang Li

Xu Chang

Yi Dong

Mingquan Wang

Jianfei Yang

Guanghui Hu

Jin Shumei

Affiliations

Soon will be listed here.

Abstract

A nitrate transporter gene, named , from salt-tolerant L. B46 has been cloned. B46NRT2.1 contained the same domain belonging to the major facilitator superfamily (PLN00028). The results of the phylogenetic tree indicated that B46NRT2.1 exhibits sequence similarity and the closest relationship with those known nitrate transporters of the NRT2 family. Through RT-qPCR, we found that the expression of mainly happens in the root and leaf. Moreover, the treatment with NaCl, NaCO, and NaHCO could significantly increase the expression of . B46NRT2.1 was located in the plasma membrane. Through the study of yeast and plant salt response brought by overexpression, we have preliminary knowledge that the expression of makes yeast and plants respond to salt shock. There are 10 different kinds of cis-acting regulatory elements (CRES) in the promotor sequences of gene using the PlantCARE web server to analyze. It mainly includes hormone response, abscisic acid, salicylic acid, gibberellin, methyl jasmonate, and auxin. The gene's co-expression network showed that it was co-expressed with a number of other genes in several biological pathways, including regulation of NO long-distance transit, modulation of nitrate sensing and metabolism, nitrate assimilation, and transduction of Jasmonic acid-independent wound signal. The results of this work should serve as a good scientific foundation for further research on the functions of the gene family in plants (inbred line B46), and this research adds to our understanding of the molecular mechanisms under salt tolerance.

Citing Articles

Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family under phytohormones and abiotic stresses in alfalfa (Medicago sativa).

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