Cannabinoids Accumulate in Mouse Breast Milk and Differentially Regulate Lipid Composition and Lipid Signaling Molecules Involved in Infant Development

Overview

Journal BBA Adv

Specialty Biochemistry

Date 2023 Jan 16

PMID 36643901

Authors

Clare T Johnson

Gabriel H Dias de Abreu

Ken Mackie

Hui-Chen Lu

Heather B Bradshaw

Affiliations

Soon will be listed here.

Abstract

Maternal cannabis use during lactation may expose developing infants to cannabinoids (CBs) such as Δ-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). CBs modulate lipid signaling molecules in the central nervous system in age- and cell-dependent ways, but their influence on the lipid composition of breast milk has yet to be established. This study investigates the effects of THC, CBD, or their combination on milk lipids by analyzing the stomach contents of CD1 mouse pups that have been nursed by dams injected with CBs on postnatal days (PND) 1 -10. Stomach contents were collected 2 hours after the last injection on PND10 and HPLC/MS/MS was used to identify and quantify over 80 endogenous lipid species and cannabinoids in the samples. We show that CBs differentially accumulate in milk, lead to widespread decreases in free fatty acids, decreases in -acyl methionine species, increases -linoleoyl species, as well as modulate levels of endogenous CBs (eCBs) AEA, 2-AG, and their structural congeners. Our data indicate the passage of CBs to pups through breast milk and that maternal CB exposure alters breast milk lipid compositions.

Citing Articles

Impact of prenatal exposure to delta 9-tetrahydrocannabinol and cannabidiol on birth size and postnatal growth trajectories.

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PMID: 39681476 PMC: 11669837. DOI: 10.1111/ijpo.13187.

Prenatal broad-spectrum cannabidiol administration prevents an autism-like phenotype in male offspring from a maternal stress/terbutaline rat model.

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PMID: 39170798 PMC: 11337682. DOI: 10.1016/j.bbih.2024.100828.

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