Efficient Separation of C-Tetramethylcalix[4]resorcinarene Conformers by Means of Reversed-Phase Solid-Phase Extraction

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2023 Jan 16

PMID 36643487

Authors

Hector Manuel Pineda-Castaneda

Mauricio Maldonado

Zuly Jenny Rivera-Monroy

Affiliations

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Abstract

A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to study the conformer formation generated during the reaction for obtaining -tetramethylcalix[4]resorcinarene. The chromatographic method was used to design a strategy for purifying the reaction products, using solid-phase extraction columns (RP-SPE) and gradient elution. The chromatographic profiles of the cyclocondensation reaction between resorcinol and acetaldehyde show the presence of three products under the different reaction and precipitation conditions studied. Using RP-SPE, it was possible to enrich the products, which were later characterized by means of RP-HPLC and H nuclear magnetic resonance (NMR). This investigation explored and established a new method for RP-HPLC analysis and RP-SPE separation of conformational isomers obtained in the formation reaction of -tetramethylcalix[4]resorcinarene.

Citing Articles

Thermally-induced atropisomerism promotes metal-organic cage construction.

Liang J, Lu S, Yang Y, Shen Y, Bai J, Sun X Nat Commun. 2023; 14(1):8166.

PMID: 38071355 PMC: 10710450. DOI: 10.1038/s41467-023-43756-4.

Peptide-Resorcinarene Conjugates Obtained via Click Chemistry: Synthesis and Antimicrobial Activity.

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