Prx4 Acts As DAMP in Shrimp, Enhancing Bacterial Resistance Via the Toll Pathway and Prophenoloxidase Activation

Overview

Journal iScience

Publisher Cell Press

Date 2023 Jan 9

PMID 36619979

Authors

Supitcha Wanvimonsuk

Phattarunda Jaree

Taro Kawai

Kunlaya Somboonwiwat

Affiliations

Soon will be listed here.

Abstract

Peroxiredoxin (Prx), an antioxidant enzyme family, has been identified as immune modulating damage-associated molecular patterns (DAMPs) in mammals but not in shrimp. Acute non-lethal heat shock (NLHS) that enhances shrimp resistance to causing acute hepatopancreatic necrosis disease (VP). Among the five Prxs (Prx) isoforms, Prx4, the most abundant in unchallenged shrimp hemocytes that was upregulated in hemocytes following NLHS treatment, is of great interest. The escalation of the Prx4 monomer in hemolymph of NLHS treated shrimp indicates that it probably acts as DAMP. This study revealed that pre-challenge with rPrx4 could prolong VP-infected shrimp survival, increase prophenoloxidase (proPO) activity and promote Toll pathway-related genes expression mediated by Toll-like receptor (TLR) 1 and 2. The presented findings elucidated the molecular mechanism of Prx4 monomer as DAMP in NLHS-induced VP resistance by inducing the TLR1/2 signaling pathway and the proPO activating system.

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