Functional Characterization of Three Diterpene Synthases Responsible for Tetracyclic Diterpene Biosynthesis in

Overview

Journal Plants (Basel)

Date 2023 Jan 8

PMID 36616198

Authors

Jung-Bum Lee

Tomoya Ohmura

Yoshimi Yamamura

Affiliations

Soon will be listed here.

Abstract

produces unique biologically active diterpenoids such as scopadulcic acid B (SDB). They are biosynthesized from geranylgeranyl diphosphate (GGPP) via -copalyl diphosphate (-CPP) and scopadulanol as an important key intermediate. In this paper, we functionally characterized three diterpene synthases, SdCPS2, SdKSL1 and SdKSL2, from The SdCPS2 catalyzed a cyclization reaction from GGPP to CPP, and SdKSL1 did from CPP to scopadulan-13α-ol. On the other hand, SdKSL2 was found to incorporate a non-sense mutation at 682. Therefore, we mutated the nucleotide residue from A to G in SdKSL2 to produce SdKSL2mut, and it was able to recover the catalytic function from -CPP to -aphidicol-16-ene, the precursor to scopadulin. From our results, SdCPS2 and SdKSL1 might be important key players for SDB biosynthesis in .

Citing Articles

Sequence-Structure Analysis Unlocking the Potential Functional Application of the Local 3D Motifs of Plant-Derived Diterpene Synthases.

Zhao Y, Liang Y, Luo G, Li Y, Han X, Wen M Biomolecules. 2024; 14(1).

PMID: 38254720 PMC: 10813164. DOI: 10.3390/biom14010120.

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