Antibacterial Thiopeptide GE2270-Congeners from

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Jan 8

PMID 36615295

Authors

Kuan-Chieh Ching

Elaine J Chin

Mario Wibowo

Zann Y Tan

Lay-Kien Yang

Deborah C Seow

Chung-Yan Leong

Veronica W Ng

Siew-Bee Ng

Yoganathan Kanagasundaram

Affiliations

Soon will be listed here.

Abstract

Thiopeptides are macrocyclic natural products with potent bioactivity. Nine new natural thiopeptides (1−9) were obtained from a Nonomuraea jiangxiensis isolated from a terrestrial soil sample collected in Singapore. Even though some of these compounds were previously synthesized or isolated from engineered strains, herein we report the unprecedented isolation of these thiopeptides from a native Nonomuraea jiangxiensis. A comparison with the literature and a detailed analysis of the NMR and HRMS of compounds 1−9 was conducted to assign their chemical structures. The structures of all new compounds were highly related to the thiopeptide antibiotics GE2270, with variations in the substituents on the thiazole and amino acid moieties. Thiopeptides 1−9 exhibited a potent antimicrobial activity against the Gram-positive bacteria, Staphylococcus aureus with MIC90 values ranging from 2 µM to 11 µM. In addition, all compounds were investigated for their cytotoxicity against the human cancer cell line A549, none of the compounds were cytotoxic.

Citing Articles

Natural Products from Singapore Soil-Derived Family and Evaluation of Their Biological Activities.

Chin E, Ching K, Tan Z, Wibowo M, Leong C, Yang L Molecules. 2023; 28(15).

PMID: 37570802 PMC: 10421265. DOI: 10.3390/molecules28155832.

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