Dynamics of Changes in Apical Cell Area During Sex Comb Rotation in

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2023 Jan 6

PMID 36606080

Authors

Aaron Doerksen

Megan Mulder

Stephen Ingram

David Nelson

Jasmine Defehr

Eleanor Reimer

Joel Atallah

Juan Nicolas Malagon

Affiliations

Soon will be listed here.

Abstract

Epithelia are highly dynamic tissues displaying various types of tissue rearrangements (Weliky and Oster, 1990; Taylor and Adler, 2008; Harris and Tepass, 2010; Lee , 2013; Firmino , 2016; Rupprecht , 2017). Here, we describe the dynamics of changes in apical cell area (ACA) in an epithelial system displaying tissue rearrangement resulting in sex comb rotation on the forelegs of male . The sex comb is a row of leg bristles which rotates during morphogenesis (Atallah, 2008; Atallah , 2009; Malagon, 2013). We quantified the ACA in the region proximal to the developing sex comb by tracing apical cell boundaries using in pupal first leg imaginal discs. We found that cells display intricate irregular oscillations in size as the comb rotates. However, the net changes in ACA within most of the cells studied are subtle, only 0 to +/-15%. Our current working hypothesis suggests these irregular oscillations confer flexibility during tissue rearrangement and can be an important mechanism for tissue homeostasis.

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