Chloroacetate Esterase Reaction Combined with Immunohistochemical Characterization of the Specific Tissue Microenvironment of Mast Cells

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2023 Jan 4

PMID 36598563

Authors

Dmitri Atiakshin

Andrey Kostin

Igor Buchwalow

Denis Morozow

Vera Samoilova

Markus Tiemann

Affiliations

Soon will be listed here.

Abstract

This study provides a combined histochemical method for detecting enzyme activity of chloroacetate esterase simultaneously with immunolabeling of the components of a specific tissue microenvironment on formalin-fixed, paraffin-embedded specimens. Chromogenic detection of the molecular targets within and outside the mast cells provides novel options in determining the histoarchitectonics of organ-specific mast cell populations, studying the functional significance of chloroacetate esterase and specifying the immune landscape of the tissue microenvironment.

Citing Articles

Mast Cells in Regeneration of the Skin in Burn Wound with Special Emphasis on Molecular Hydrogen Effect.

Atiakshin D, Soboleva M, Nikityuk D, Alexeeva N, Klochkova S, Kostin A Pharmaceuticals (Basel). 2023; 16(3).

PMID: 36986447 PMC: 10059032. DOI: 10.3390/ph16030348.

References

Atiakshin D, Kostin A, Trotsenko I, Samoilova V, Buchwalow I, Tiemann M . Carboxypeptidase A3-A Key Component of the Protease Phenotype of Mast Cells. Cells. 2022; 11(3). PMC: 8834431. DOI: 10.3390/cells11030570. View

Wong E, Morgan E, Macdonald D . The chloroacetate esterase reaction for mast cells in dermatopathology: a comparison with metachromatic staining methods. Acta Derm Venereol. 1982; 62(5):431-4. View

Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M . Characterization of mast cell populations using different methods for their identification. Histochem Cell Biol. 2017; 147(6):683-694. DOI: 10.1007/s00418-017-1547-7. View

Gomori G . Chloroacyl esters as histochemical substrates. J Histochem Cytochem. 1953; 1(6):469-70. DOI: 10.1177/1.6.469. View

Rindler R, Schmalzl F, Hortnagl H, BRAUNSTEINER H . Naphthol AS-D chloroacetate esterases in granule extracts from human neutrophil leukocytes. Blut. 1971; 23(4):223-7. DOI: 10.1007/BF01633774. View

BENDITT E, ARASE M . An enzyme in mast cells with properties like chymotrypsin. J Exp Med. 1959; 110:451-60. PMC: 2137016. DOI: 10.1084/jem.110.3.451. View

Michova M, Fakan F . [Cytochemical and histochemical determination of naphthol-AS-D-chloroacetate esterase and its use in diagnosis]. Lab Delo. 1980; (7):393-5. View

Uchihara Y, Inagaki Y, Munemoto M, Tanaka Y, Athanasou N . Histological Findings in Infected and Noninfected Second Stage Revision Knee Arthroplasties. J Knee Surg. 2018; 32(10):1015-1019. DOI: 10.1055/s-0038-1675341. View

Atiakshin D, Buchwalow I, Samoilova V, Tiemann M . Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol. 2018; 149(5):461-477. DOI: 10.1007/s00418-018-1659-8. View

10.

Kashima T, Inagaki Y, Grammatopoulos G, Athanasou N . Use of chloroacetate esterase staining for the histological diagnosis of prosthetic joint infection. Virchows Arch. 2015; 466(5):595-601. DOI: 10.1007/s00428-015-1722-y. View

11.

MOLONEY W, McPherson K, FLIEGELMAN L . Esterase activity in leukocytes demonstrated by the use of naphthol AS-D chloroacetate substrate. J Histochem Cytochem. 1960; 8:200-7. DOI: 10.1177/8.3.200. View

12.

Hahn von Dorsche H, Stiller D, PAMBOR M, Schwesinger G, Sodemann S, STOLP A . [Occurrence of naphthol-AS-D-chloracetate-esterase-activity in mast cells with special references to cutaneous mastocytoses (author's transl)]. Acta Histochem. 1981; 69(1):23-30. View

13.

Milicevic N, Milicevic Z . Naphthol AS D chloroacetate esterase-positive macrophages in the cortico-medullary zone of the normal rat thymus. Virchows Arch B Cell Pathol Incl Mol Pathol. 1985; 50(2):193-8. DOI: 10.1007/BF02889901. View

14.

Chinprasertsuk S, Piankijagum A, Issaragrisil S . Cytochemical stainings in acute promyelocytic leukemia: chloroacetate esterase reaction as a prognostic index. J Med Assoc Thai. 1991; 74(2):87-91. View

15.

Buchwalow I, Samoilova V, Boecker W, Tiemann M . Non-specific binding of antibodies in immunohistochemistry: fallacies and facts. Sci Rep. 2012; 1:28. PMC: 3216515. DOI: 10.1038/srep00028. View

16.

Atiakshin D, Buchwalow I, Tiemann M . Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol. 2019; 152(4):253-269. DOI: 10.1007/s00418-019-01803-6. View

17.

Glenner G, COHEN L . Histochemical demonstration of a species-specific trypsin-like enzyme in mast cells. Nature. 1960; 185:846-7. DOI: 10.1038/185846a0. View

18.

Li C, Lam K, Yam L . Esterases in human leukocytes. J Histochem Cytochem. 1973; 21(1):1-12. DOI: 10.1177/21.1.1. View

19.

Rindler R, Hortnagl H, Schmalzl F, BRAUNSTEINER H . Hydrolysis of a chymotrypsin substrate and of naphthol AS-D chloroacetate by human leukocyte granules. Blut. 1973; 26(4):239-49. DOI: 10.1007/BF01631788. View

20.

Wang K, Feng B, Wang G, Cui J, Yang L, Jiang K . A specific esterase and pH logically regulate ESIPT: different kinds of granulocyte sorting. Chem Commun (Camb). 2022; 58(17):2894-2897. DOI: 10.1039/d1cc07261g. View