Scattering Angle Resolved Optical Coherence Tomography Measures Morphological Changes in Colonies

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2023 Jan 2

PMID 36590979

Authors

Vikram Barauah

Shyon Parsa

Naail Chowdhury

Thomas Milner

Henry Grady Rylander 3rd

Affiliations

Soon will be listed here.

Abstract

Significance: An unmet need is recognized for early detection and diagnosis of neurological diseases. Many psychological markers emerge years after disease onset. Mitochondrial dysfunction and corresponding neurodegeneration occur before onset of large-scale cell and tissue pathology. Early detection of subcellular morphology changes could serve as a beacon for early detection of neurological diseases. This study is on bacterial colonies, , which are similar in size to mitochondria.

Aim: This study investigates whether morphological changes can be detected in using scattering angle resolved optical coherence tomography (SAR-OCT).

Approach: The SAR-OCT was applied to detect scattering angle distribution changes in The rod-to-coccus shape transition of the bacteria was imaged, and the backscattering angle was analyzed by recording the distribution of the ratio of low- to medium angle scattering (L/M ratio). orientation at different locations in colonies was analytically modeled and compared with SAR-OCT results.

Results: Significant differences in the distribution of backscattering angle were observed in transitioning from rod-to-coccus shapes. In , the -parameter of the Burr distribution of the SAR-OCT-derived L/M ratio was significantly smaller in coccus compared with rod-shaped bacteria. SAR-OCT-derived L/M ratio varied with bacterial position in the colony and is consistent with predicted orientations from previous studies.

Conclusions: Study results support the potential of utilizing SAR-OCT to detect bacterial morphological changes.

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