The Concentrations of Bone Calcium, Phosphorus and Trace Metal Elements in Elderly Patients with Intertrochanteric Hip Fractures

Overview

Journal Front Endocrinol (Lausanne)

Specialty Endocrinology

Date 2022 Dec 30

PMID 36582999

Authors

Shangjin Lin

Cong Chen

Xiaoxi Cai

Fengjian Yang

Yongqian Fan

Affiliations

Soon will be listed here.

Abstract

Introduction: Trace metal elements may play a crucial role in bone mineralization and metabolism. However, the quantification of trace element concentrations in human bone tissue has received little attention.

Materials And Methods: Bone tissue samples were collected from 55 elderly patients (15 males and 40 females) with intertrochanteric hip fractures. The calcium, phosphorus, manganese, iron, copper, and zinc concentrations in the cortical bone zone, cancellous bone zone, and junction zone between cortical and cancellous bone were determined by energy-dispersive X-ray fluorescence (EDX). The differences in trace element concentrations in the three regions were compared, and the correlation between gender and bone trace element contents of the bones was analyzed using the Kruskal-Wallis's test. The correlation between age, body mass index (BMI), and bone calcium, phosphorus concentrations, and trace elements in three bone zones was determined using Spearman correlation analysis.

Results: The Kruskal-Wallis test showed no difference in bone phosphorus concentration among the three regions. In contrast, the difference in the concentrations of bone calcium and four metal elements was statistically significant (<0.01). In addition, no statistical differences were observed in the concentrations of trace elements among the three regions in elderly male and female patients. Spearman correlation analysis showed a strong negative correlation between bone calcium and phosphorus in three bone regions (r=-0.999, -0.95, -0.998, < 0.01) and a significant positive correlation between trace metal elements in the cancellous bone zone. In the junction zone, the BMI showed a strong positive correlation with bone calcium content (r=0.347, =0.009) and a significant negative correlation with phosphorus content (r=-0.349, =0.009).

Conclusion: Bone calcium and phosphorus were the main components of hydroxyapatite, and these two elements accounted for the majority of bone mineral salts. Trace metal elements are essential for bone metabolism and specific synergistic interactions. BMI may be associated with bone calcium and phosphorus contents in elderly patients with osteoporosis.

Citing Articles

Diversity of the diet is correlated with osteoporosis in post-menopausal women: an Iranian case-control study.

Abbasi B, Hajinasab M, Mohammadi Zadeh Z, Ahmadi P Front Public Health. 2024; 12:1431181.

PMID: 39185118 PMC: 11342058. DOI: 10.3389/fpubh.2024.1431181.

References

Pemmer B, Roschger A, Wastl A, Hofstaetter J, Wobrauschek P, Simon R . Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue. Bone. 2013; 57(1):184-93. PMC: 3807669. DOI: 10.1016/j.bone.2013.07.038. View

Wang L, Yu H, Yang G, Wang W, Su T, Ma W . Correlation between bone mineral density and serum trace element contents of elderly males in Beijing urban area. Int J Clin Exp Med. 2016; 8(10):19250-7. PMC: 4694461. View

Ceylan M, Akdas S, Yazihan N . Is Zinc an Important Trace Element on Bone-Related Diseases and Complications? A Meta-analysis and Systematic Review from Serum Level, Dietary Intake, and Supplementation Aspects. Biol Trace Elem Res. 2020; 199(2):535-549. DOI: 10.1007/s12011-020-02193-w. View

Duck K, Connor J . Iron uptake and transport across physiological barriers. Biometals. 2016; 29(4):573-91. PMC: 4972853. DOI: 10.1007/s10534-016-9952-2. View

Li Y, Hui M, Chang X, Li M, Wang Y, Zhang B . BMI reduction and vitamin D insufficiency mediated osteoporosis and fragility fractures in patients at nutritional risk: a cross-sectional study. Eur J Clin Nutr. 2018; 72(3):455-459. DOI: 10.1038/s41430-017-0067-9. View

Zaichick V, Zaichick S, Karandashev V, Nosenko S . The effect of age and gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn contents in rib bone of healthy humans. Biol Trace Elem Res. 2008; 129(1-3):107-15. DOI: 10.1007/s12011-008-8302-9. View

Dermience M, Lognay G, Mathieu F, Goyens P . Effects of thirty elements on bone metabolism. J Trace Elem Med Biol. 2015; 32:86-106. DOI: 10.1016/j.jtemb.2015.06.005. View

Liu S, Yan H, Xu P, Li J, Zhuang G, Zhu B . Correlation analysis between bone mineral density and serum element contents of postmenopausal women in Xi'an urban area. Biol Trace Elem Res. 2009; 131(3):205-14. DOI: 10.1007/s12011-009-8363-4. View

Ang S, Xia J, Cheng S, Chua M, Goh L, Dhaliwal S . A pilot screening study for low bone mass in Singaporean women using years since menopause and BMI. Climacteric. 2021; 25(2):163-169. DOI: 10.1080/13697137.2021.1908989. View

10.

Kuo H, Kuo S, Chou C, Lee T . Determination of 14 elements in Taiwanese bones. Sci Total Environ. 2000; 255(1-3):45-54. DOI: 10.1016/s0048-9697(00)00448-4. View

11.

Brodziak-Dopierala B, Kwapulinski J, Kusz D, Gajda Z, Sobczyk K . Interactions between concentrations of chemical elements in human femoral heads. Arch Environ Contam Toxicol. 2008; 57(1):203-10. DOI: 10.1007/s00244-008-9228-0. View

12.

Blanco-Rojo R, Perez-Granados A, Toxqui L, Zazo P, Piedra C, Vaquero M . Relationship between vitamin D deficiency, bone remodelling and iron status in iron-deficient young women consuming an iron-fortified food. Eur J Nutr. 2012; 52(2):695-703. DOI: 10.1007/s00394-012-0375-8. View

13.

Da Silva T, Hiller C, Gai Z, Kullak-Ublick G . Vitamin D3 transactivates the zinc and manganese transporter SLC30A10 via the Vitamin D receptor. J Steroid Biochem Mol Biol. 2016; 163:77-87. DOI: 10.1016/j.jsbmb.2016.04.006. View

14.

Brodziak-Dopierala B, Kwapulinski J, Sobczyk K, Wiechula D . The content of manganese and iron in hip joint tissue. J Trace Elem Med Biol. 2013; 27(3):208-12. DOI: 10.1016/j.jtemb.2012.12.005. View

15.

Drzazga Z, Michalik K, Maciejewska K, Trzeciak H, Kaszuba M . Role of endogenous zinc in bones of newborn rats. Biofactors. 2008; 30(4):243-8. DOI: 10.1002/biof.5520300405. View

16.

Budis H, Kalisinska E, Lanocha N, Kosik-Bogacka D, Sokolowski S, Dobiecki K . The concentration of manganese, iron, and strontium in hip joint bone obtained from patients undergoing hip replacement surgery. J Trace Elem Med Biol. 2013; 28(1):39-44. DOI: 10.1016/j.jtemb.2013.07.004. View

17.

Chon S, Koh Y, Heo J, Lee J, Kim M, Yun B . Effects of vitamin D deficiency and daily calcium intake on bone mineral density and osteoporosis in Korean postmenopausal woman. Obstet Gynecol Sci. 2017; 60(1):53-62. PMC: 5313364. DOI: 10.5468/ogs.2017.60.1.53. View

18.

Lanocha N, Kalisinska E, Kosik-Bogacka D, Budis H, Sokolowski S, Bohatyrewicz A . Concentrations of trace elements in bones of the hip joint from patients after hip replacement surgery. J Trace Elem Med Biol. 2012; 26(1):20-5. DOI: 10.1016/j.jtemb.2011.11.006. View

19.

Milkovic L, Hoppe A, Detsch R, Boccaccini A, Zarkovic N . Effects of Cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cells in vitro. J Biomed Mater Res A. 2013; 102(10):3556-61. DOI: 10.1002/jbm.a.35032. View

20.

Roczniak W, Brodziak-Dopierala B, Cipora E, Jakobik-Kolon A, Kluczka J, Babuska-Roczniak M . Factors that Affect the Content of Cadmium, Nickel, Copper and Zinc in Tissues of the Knee Joint. Biol Trace Elem Res. 2017; 178(2):201-209. PMC: 5506214. DOI: 10.1007/s12011-016-0927-5. View