The Potential of Cold-shock Promoters for the Expression of Recombinant Proteins in Microbes and Mammalian Cells

Overview

Journal J Genet Eng Biotechnol

Specialty Biotechnology

Date 2022 Dec 29

PMID 36580173

Authors

Yaneth Bartolo-Aguilar

Cipriano Chavez-Cabrera

Luis Bernardo Flores-Cotera

Jesus Agustin Badillo-Corona

Carmen Oliver-Salvador

Rodolfo Marsch

Affiliations

Soon will be listed here.

Abstract

Background: Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells.

Conclusions: This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express.

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