Lactiplantibacillus Plantarum PGB02 Improved Serum Cholesterol Profile by Tweaking Genes Involved in Cholesterol Homeostasis in Male Swiss Albino Mice

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2022 Dec 27

PMID 36574136

Authors

Hiren A Dhameliya

Jyoti D Solanki

Dhaval T Patel

Linz-Buoy George

Vasudev R Thakkar

R B Subramanian

Affiliations

Soon will be listed here.

Abstract

The effect of Lactiplantibacillus plantarum PGB02 isolated from buttermilk on serum cholesterol profile of normal and hypercholesterolemic mice was evaluated. Further changes in the expression of mice genes were determined. The hypercholesterolemia was induced in experimental mice by feeding high cholesterol and fat diet. Serum cholesterol parameters, physical parameters, cholic acid excretion, and cholesterol metabolism related gene expression analysis was carried out. L. plantarum PGB02 efficiently reduced total cholesterol, triglycerides, and LDL-cholesterol and improved HDL-cholesterol in hypercholesterolaemic mice. Body weight was reduced and fecal cholic acid increased in probiotic treatment groups. Gene expression analysis revealed that L. plantarum PGB02 up-regulated the expression of LDL receptors, CYP7A1, ABCA1, ABCG5, ABCG8, and down-regulated the expression of FXR and NPC1L1 genes. Summarizing the mechanism, L. plantarum PGB02 improved hypercholesterolemia by increasing bile acid synthesis and excretion, reducing exogeneous cholesterol absorption from the intestine, and increased LDL clearance through upregulation of LDL-receptors. The present study has given insight into the mechanism of serum cholesterol reduction by bile salt hydrolase positive L. plantarum PGB02 in mice. L. plantarum PGB02 reduced the serum cholesterol level through increased bile acid synthesis and deconjugation and reduced absorption of cholesterol in the intestine. Isolate PGB02 shown cholesterol removal potential as good as statin.

Citing Articles

Effects of Lactiplantibacillus plantarum CBT LP3 and Bifidobacterium breve CBT BR3 supplementation on weight loss and gut microbiota of overweight dogs.

Choi J, Son D, An S, Cho E, Lim S, Lee H Sci Rep. 2024; 14(1):25446.

PMID: 39455650 PMC: 11511819. DOI: 10.1038/s41598-024-75594-9.

References

Banach M, Rizzo M, Toth P, Farnier M, Davidson M, Al-Rasadi K . Statin intolerance - an attempt at a unified definition. Position paper from an International Lipid Expert Panel. Arch Med Sci. 2015; 11(1):1-23. PMC: 4379380. DOI: 10.5114/aoms.2015.49807. View

Kumar M, Rakesh S, Nagpal R, Hemalatha R, Ramakrishna A, Sudarshan V . Probiotic Lactobacillus rhamnosus GG and Aloe vera gel improve lipid profiles in hypercholesterolemic rats. Nutrition. 2013; 29(3):574-9. DOI: 10.1016/j.nut.2012.09.006. View

Liong M, Shah N . Effects of a Lactobacillus casei synbiotic on serum lipoprotein, intestinal microflora, and organic acids in rats. J Dairy Sci. 2006; 89(5):1390-9. DOI: 10.3168/jds.S0022-0302(06)72207-X. View

Pereira D, Gibson G . Effects of consumption of probiotics and prebiotics on serum lipid levels in humans. Crit Rev Biochem Mol Biol. 2002; 37(4):259-81. DOI: 10.1080/10409230290771519. View

Choi S, Lew L, Yeo S, Parvathy S, Liong M . Probiotics and the BSH-related cholesterol lowering mechanism: a Jekyll and Hyde scenario. Crit Rev Biotechnol. 2014; 35(3):392-401. DOI: 10.3109/07388551.2014.889077. View

Lew L, Choi S, Khoo B, Sreenivasan S, Ong K, Liong M . DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase. Korean J Food Sci Anim Resour. 2018; 38(2):350-361. PMC: 5960832. DOI: 10.5851/kosfa.2018.38.2.350. View

Huang Y, Zheng Y . The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann-Pick C1-like 1 in Caco-2 cells. Br J Nutr. 2009; 103(4):473-8. DOI: 10.1017/S0007114509991991. View

FRIEDEWALD W, Levy R, Fredrickson D . Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18(6):499-502. View

Kumar R, Grover S, Batish V . Hypocholesterolaemic effect of dietary inclusion of two putative probiotic bile salt hydrolase-producing Lactobacillus plantarum strains in Sprague-Dawley rats. Br J Nutr. 2010; 105(4):561-73. DOI: 10.1017/S0007114510003740. View

10.

Jeun J, Kim S, Cho S, Jun H, Park H, Seo J . Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition. 2009; 26(3):321-30. DOI: 10.1016/j.nut.2009.04.011. View

11.

Clayton P . Disorders of cholesterol biosynthesis. Arch Dis Child. 1998; 78(2):185-9. PMC: 1717457. DOI: 10.1136/adc.78.2.185. View

12.

McCrindle B, Ose L, Marais A . Efficacy and safety of atorvastatin in children and adolescents with familial hypercholesterolemia or severe hyperlipidemia: a multicenter, randomized, placebo-controlled trial. J Pediatr. 2003; 143(1):74-80. DOI: 10.1016/S0022-3476(03)00186-0. View

13.

Silverman M, Ference B, Im K, Wiviott S, Giugliano R, Grundy S . Association Between Lowering LDL-C and Cardiovascular Risk Reduction Among Different Therapeutic Interventions: A Systematic Review and Meta-analysis. JAMA. 2016; 316(12):1289-97. DOI: 10.1001/jama.2016.13985. View

14.

Chand D, Avinash V, Yadav Y, Pundle A, Suresh C, Ramasamy S . Molecular features of bile salt hydrolases and relevance in human health. Biochim Biophys Acta Gen Subj. 2016; 1861(1 Pt A):2981-2991. DOI: 10.1016/j.bbagen.2016.09.024. View

15.

Cohen J, Cali J, Jelinek D, Mehrabian M, Sparkes R, Lusis A . Cloning of the human cholesterol 7 alpha-hydroxylase gene (CYP7) and localization to chromosome 8q11-q12. Genomics. 1992; 14(1):153-61. DOI: 10.1016/s0888-7543(05)80298-8. View

16.

Bell G, Lewis B, Petrie A, Dowling R . Serum lipids in cholelithiasis: effect of chenodeoxycholic acid therapy. Br Med J. 1973; 3(5879):520-3. PMC: 1586970. DOI: 10.1136/bmj.3.5879.520. View

17.

Bateson M, Maclean D, Evans J, Bouchier I . Chenodeoxycholic acid therapy for hypertriglyceridaemia in men. Br J Clin Pharmacol. 1978; 5(3):249-54. PMC: 1429265. DOI: 10.1111/j.1365-2125.1978.tb01632.x. View

18.

Crouse 3rd J . Hypertriglyceridemia: a contraindication to the use of bile acid binding resins. Am J Med. 1987; 83(2):243-8. DOI: 10.1016/0002-9343(87)90692-9. View

19.

Beil U, Crouse J, Einarsson K, Grundy S . Effects of interruption of the enterohepatic circulation of bile acids on the transport of very low density-lipoprotein triglycerides. Metabolism. 1982; 31(5):438-44. DOI: 10.1016/0026-0495(82)90231-1. View

20.

Young S, Fielding C . The ABCs of cholesterol efflux. Nat Genet. 1999; 22(4):316-8. DOI: 10.1038/11878. View