Plasmodium Falciparum Pfhrp2 and Pfhrp3 Gene Deletions in Malaria-Hyperendemic Region, South Sudan

Overview

Journal Emerg Infect Dis

Specialty Infectious Diseases

Date 2022 Dec 27

PMID 36573593

Authors

Irene Molina-de la Fuente

Maria Jose Sagrado Benito

Laurence Flevaud

Janet Ousley

Harriet Akello Pasquale

Ahmed Julla

Abdirashid M Abdi

Buai Tut Chol

Bakri Abubakr

Agustin Benito

Cristian Casademont

Carolina Nanclares

Pedro Berzosa

Affiliations

Soon will be listed here.

Abstract

Pfhrp2 and pfhrp3 gene deletions threaten the use of Plasmodium falciparum malaria rapid diagnostic tests globally. In South Sudan, deletion frequencies were 15.6% for pfhrp2, 20.0% for pfhrp3, and 7.5% for double deletions. Deletions were approximately twice as prevalent in monoclonal infections than in polyclonal infections.

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References

Agaba B, Anderson K, Gresty K, Prosser C, Smith D, Nankabirwa J . Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017-2019. Malar J. 2020; 19(1):300. PMC: 7449024. DOI: 10.1186/s12936-020-03362-x. View

Prosser C, Gresty K, Ellis J, Meyer W, Anderson K, Lee R . Plasmodium falciparum Histidine-Rich Protein 2 and 3 Gene Deletions in Strains from Nigeria, Sudan, and South Sudan. Emerg Infect Dis. 2021; 27(2):471-479. PMC: 7853540. DOI: 10.3201/eid2702.191410. View

Parr J, Verity R, Doctor S, Janko M, Carey-Ewend K, Turman B . Pfhrp2-Deleted Plasmodium falciparum Parasites in the Democratic Republic of the Congo: A National Cross-sectional Survey. J Infect Dis. 2017; 216(1):36-44. PMC: 6279174. DOI: 10.1093/infdis/jiw538. View

Boush M, Djibrine M, Mussa A, Talib M, Maki A, Mohammed A . Plasmodium falciparum isolate with histidine-rich protein 2 gene deletion from Nyala City, Western Sudan. Sci Rep. 2020; 10(1):12822. PMC: 7393171. DOI: 10.1038/s41598-020-69756-8. View

Rogier E, McCaffery J, Nace D, Svigel S, Assefa A, Hwang J . Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions from Persons with Symptomatic Malaria Infection in Ethiopia, Kenya, Madagascar, and Rwanda. Emerg Infect Dis. 2022; 28(3):608-616. PMC: 8888236. DOI: 10.3201/eid2803.211499. View

Watson O, Slater H, Verity R, Parr J, Mwandagalirwa M, Tshefu A . Modelling the drivers of the spread of gene deletions in sub-Saharan Africa. Elife. 2017; 6. PMC: 5602420. DOI: 10.7554/eLife.25008. View

Grignard L, Nolder D, Sepulveda N, Berhane A, Mihreteab S, Kaaya R . A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections. EBioMedicine. 2020; 55:102757. PMC: 7218259. DOI: 10.1016/j.ebiom.2020.102757. View

Sondo P, Derra K, Rouamba T, Nakanabo Diallo S, Taconet P, Kazienga A . Determinants of Plasmodium falciparum multiplicity of infection and genetic diversity in Burkina Faso. Parasit Vectors. 2020; 13(1):427. PMC: 7441709. DOI: 10.1186/s13071-020-04302-z. View

Pinkevych M, Petravic J, Bereczky S, Rooth I, Farnert A, Davenport M . Understanding the relationship between Plasmodium falciparum growth rate and multiplicity of infection. J Infect Dis. 2014; 211(7):1121-7. DOI: 10.1093/infdis/jiu561. View

10.

Beshir K, Sepulveda N, Bharmal J, Robinson A, Mwanguzi J, Busula A . Plasmodium falciparum parasites with histidine-rich protein 2 (pfhrp2) and pfhrp3 gene deletions in two endemic regions of Kenya. Sci Rep. 2017; 7(1):14718. PMC: 5677122. DOI: 10.1038/s41598-017-15031-2. View

11.

Feleke S, Reichert E, Mohammed H, Brhane B, Mekete K, Mamo H . Plasmodium falciparum is evolving to escape malaria rapid diagnostic tests in Ethiopia. Nat Microbiol. 2021; 6(10):1289-1299. PMC: 8478644. DOI: 10.1038/s41564-021-00962-4. View

12.

Berzosa P, Gonzalez V, Taravillo L, Mayor A, Romay-Barja M, Garcia L . First evidence of the deletion in the pfhrp2 and pfhrp3 genes in Plasmodium falciparum from Equatorial Guinea. Malar J. 2020; 19(1):99. PMC: 7050119. DOI: 10.1186/s12936-020-03178-9. View