Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein

Overview

Journal ACS Appl Nano Mater

Publisher American Chemical Society

Date 2022 Dec 26

PMID 36570474

Authors

Kai Wu

Vinit Kumar Chugh

Venkatramana D Krishna

Yongqiang Andrew Wang

Timothy D Gordon

Maxim C-J Cheeran

Jian-Ping Wang

Affiliations

Soon will be listed here.

Abstract

In this work, we report a 5-min magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface-functionalized magnetic nanoparticles are incubated with target analytes at 37 °C with agitation for 3 min, and the MPS reading is then taken at the fifth minute. We prove the feasibility of 5 min ultrafast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmol). Our proposed 5-min bioassay strategy may be applied to reduce the assay time for other liquid-phase, volumetric biosensors such as NMR, quantum dots, fluorescent biosensors, etc.

Citing Articles

Impact of Particle Size on the Nonlinear Magnetic Response of Iron Oxide Nanoparticles during Frequency Mixing Magnetic Detection.

Pourshahidi A, Jean N, Kaulen C, Jakobi S, Krause H Sensors (Basel). 2024; 24(13).

PMID: 39001003 PMC: 11244231. DOI: 10.3390/s24134223.

MICaFVi: A Novel Magnetic Immuno-Capture Flow Virometry Nano-Based Diagnostic Tool for Detection of Coronaviruses.

Samman N, El-Boubbou K, Al-Muhalhil K, Ali R, Alaskar A, Alharbi N Biosensors (Basel). 2023; 13(5).

PMID: 37232914 PMC: 10216117. DOI: 10.3390/bios13050553.

References

Tian B, Liao X, Svedlindh P, Stromberg M, Wetterskog E . Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA. ACS Sens. 2018; 3(6):1093-1101. DOI: 10.1021/acssensors.8b00048. View

Wu K, Liu J, Su D, Saha R, Wang J . Magnetic Nanoparticle Relaxation Dynamics-Based Magnetic Particle Spectroscopy for Rapid and Wash-Free Molecular Sensing. ACS Appl Mater Interfaces. 2019; 11(26):22979-22986. DOI: 10.1021/acsami.9b05233. View

Wu K, Liu J, Saha R, Su D, Krishna V, Cheeran M . Magnetic Particle Spectroscopy for Detection of Influenza A Virus Subtype H1N1. ACS Appl Mater Interfaces. 2020; 12(12):13686-13697. DOI: 10.1021/acsami.0c00815. View

Gordon-Wylie S, Ness D, Shi Y, Mirza S, Paulsen K, Weaver J . Measuring protein biomarker concentrations using antibody tagged magnetic nanoparticles. Biomed Phys Eng Express. 2021; 6(6). PMC: 8528228. DOI: 10.1088/2057-1976/abc45b. View

Bragina V, Znoyko S, Orlov A, Pushkarev A, Nikitin M, Nikitin P . Analytical Platform with Selectable Assay Parameters Based on Three Functions of Magnetic Nanoparticles: Demonstration of Highly Sensitive Rapid Quantitation of Staphylococcal Enterotoxin B in Food. Anal Chem. 2019; 91(15):9852-9857. DOI: 10.1021/acs.analchem.9b01519. View

Pietschmann J, Spiegel H, Krause H, Schillberg S, Schroper F . Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection. Toxins (Basel). 2020; 12(5). PMC: 7290995. DOI: 10.3390/toxins12050337. View

Zhang X, Reeves D, Perreard I, Kett W, Griswold K, Gimi B . Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion. Biosens Bioelectron. 2013; 50:441-6. PMC: 3844855. DOI: 10.1016/j.bios.2013.06.049. View

Pietschmann J, Voepel N, Voss L, Rasche S, Schubert M, Kleines M . Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay. Front Microbiol. 2021; 12:643275. PMC: 8132704. DOI: 10.3389/fmicb.2021.643275. View

Orlov A, Znoyko S, Cherkasov V, Nikitin M, Nikitin P . Multiplex Biosensing Based on Highly Sensitive Magnetic Nanolabel Quantification: Rapid Detection of Botulinum Neurotoxins A, B, and E in Liquids. Anal Chem. 2016; 88(21):10419-10426. DOI: 10.1021/acs.analchem.6b02066. View

10.

Lee H, Sun E, Ham D, Weissleder R . Chip-NMR biosensor for detection and molecular analysis of cells. Nat Med. 2008; 14(8):869-74. PMC: 2729055. DOI: 10.1038/nm.1711. View

11.

Rettcher S, Jungk F, Kuhn C, Krause H, Nolke G, Commandeur U . Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses. Appl Environ Microbiol. 2015; 81(9):3039-48. PMC: 4393444. DOI: 10.1128/AEM.03667-14. View

12.

Zhong J, Rosch E, Viereck T, Schilling M, Ludwig F . Toward Rapid and Sensitive Detection of SARS-CoV-2 with Functionalized Magnetic Nanoparticles. ACS Sens. 2021; 6(3):976-984. DOI: 10.1021/acssensors.0c02160. View

13.

Moitra P, Alafeef M, Dighe K, Frieman M, Pan D . Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. ACS Nano. 2020; 14(6):7617-7627. DOI: 10.1021/acsnano.0c03822. View

14.

Guteneva N, Znoyko S, Orlov A, Nikitin M, Nikitin P . Rapid lateral flow assays based on the quantification of magnetic nanoparticle labels for multiplexed immunodetection of small molecules: application to the determination of drugs of abuse. Mikrochim Acta. 2019; 186(9):621. DOI: 10.1007/s00604-019-3726-9. View

15.

Cheong J, Yu H, Lee C, Lee J, Choi H, Lee J . Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device. Nat Biomed Eng. 2020; 4(12):1159-1167. PMC: 8202505. DOI: 10.1038/s41551-020-00654-0. View

16.

Wu K, Chugh V, Krishna V, di Girolamo A, Wang Y, Saha R . One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase. ACS Appl Mater Interfaces. 2021; 13(37):44136-44146. PMC: 8442556. DOI: 10.1021/acsami.1c14657. View

17.

Luo Y, Alocilja E . Portable nuclear magnetic resonance biosensor and assay for a highly sensitive and rapid detection of foodborne bacteria in complex matrices. J Biol Eng. 2017; 11:14. PMC: 5369001. DOI: 10.1186/s13036-017-0053-8. View