Development and Validation of a Predictive Model for Immune-related Genes in Patients with Tongue Squamous Cell Carcinoma

Overview

Journal Open Life Sci

Specialty Biology

Date 2022 Dec 26

PMID 36567723

Authors

Meng Yang

Changyu Zeng

Zhongcheng Gong

Bo Shao

Gaocheng Liu

Xuying Bao

Bin Nie

Affiliations

Soon will be listed here.

Abstract

The present study involved building a model of immune-related genes (IRGs) that can predict the survival outcomes of tongue squamous cell carcinoma (TSCC). Using the TCGA database, we collected the gene expression profiles of patients with TSCC and analyzed the differences in IRGs obtained from the ImmPort database. Subsequently, we constructed a predictive model. Transcription factors and differentially expressed IRGs can be used to construct TSCC regulatory network. CIBERSORT tool was used to analyze the relative proportion of 22 tumor-infiltrating immune cells in TSCC samples. Finally, a prognostic model is constructed. We established an IRG model formed by seven genes. The receiver operating characteristic value of the prognostic model based on IRGs is 0.739. After the analysis of the correlation between IRGs and clinical and pathological conditions, we found that Gast was related to grade, IRF9, LTB, and T stage. Among the 22 tumor-infiltrating immune cells, the resting natural killer (NK) cells were found to be related to the 5-year survival rate. This study constructed a prognostic model formed by seven IRGs and discussed the tumor-infiltrating immune cells, which are related to the survival outcome, reflecting the potential regulatory role of TSCC tumor immune microenvironment that could potentially promote individualized treatment.

References

Yang X, Cheng H, Chen J, Wang R, Saleh A, Si H . Head and Neck Cancers Promote an Inflammatory Transcriptome through Coactivation of Classic and Alternative NF-κB Pathways. Cancer Immunol Res. 2019; 7(11):1760-1774. PMC: 6941750. DOI: 10.1158/2326-6066.CIR-18-0832. View

Chen X, Lu K, Timko N, Weir D, Zhu Z, Qin C . IL-33 notably inhibits the growth of colon cancer cells. Oncol Lett. 2018; 16(1):769-774. PMC: 6019937. DOI: 10.3892/ol.2018.8728. View

Newman A, Liu C, Green M, Gentles A, Feng W, Xu Y . Robust enumeration of cell subsets from tissue expression profiles. Nat Methods. 2015; 12(5):453-7. PMC: 4739640. DOI: 10.1038/nmeth.3337. View

Siegel R, Miller K, Jemal A . Cancer statistics, 2019. CA Cancer J Clin. 2019; 69(1):7-34. DOI: 10.3322/caac.21551. View

Chen J, Liu L, Cai X, Yao Z, Huang J . Progress in the study of long noncoding RNA in tongue squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol. 2019; 129(1):51-58. DOI: 10.1016/j.oooo.2019.08.011. View

He R, Zhou X, Yi Q, Deng C, Gao J, Chen G . Prognostic Signature of Alternative Splicing Events in Bladder Urothelial Carcinoma Based on Spliceseq Data from 317 Cases. Cell Physiol Biochem. 2018; 48(3):1355-1368. DOI: 10.1159/000492094. View

Slezak J, Truong M, Huang W, Jarrard D . HP1γ expression is elevated in prostate cancer and is superior to Gleason score as a predictor of biochemical recurrence after radical prostatectomy. BMC Cancer. 2013; 13:148. PMC: 3618256. DOI: 10.1186/1471-2407-13-148. View

Hsu D, Hwang W, Yuh C, Chu C, Ho Y, Chen P . Lymphotoxin-β Interacts with Methylated EGFR to Mediate Acquired Resistance to Cetuximab in Head and Neck Cancer. Clin Cancer Res. 2017; 23(15):4388-4401. DOI: 10.1158/1078-0432.CCR-16-1955. View

Wan Y, Liu H, Zhang M, Huang Z, Zhou H, Zhu Y . Prognostic value of epithelial-mesenchymal transition-inducing transcription factors in head and neck squamous cell carcinoma: A meta-analysis. Head Neck. 2020; 42(5):1067-1076. DOI: 10.1002/hed.26104. View

10.

Wu Y, Bian C, Zhen C, Liu L, Lin Z, Nisar M . Telomerase reverse transcriptase mediates EMT through NF-κB signaling in tongue squamous cell carcinoma. Oncotarget. 2017; 8(49):85492-85503. PMC: 5689625. DOI: 10.18632/oncotarget.20888. View

11.

Mei S, Meyer C, Zheng R, Qin Q, Wu Q, Jiang P . Cistrome Cancer: A Web Resource for Integrative Gene Regulation Modeling in Cancer. Cancer Res. 2017; 77(21):e19-e22. PMC: 5826647. DOI: 10.1158/0008-5472.CAN-17-0327. View

12.

Weng J, Zhang H, Wang C, Liang J, Chen G, Li W . miR-373-3p Targets DKK1 to Promote EMT-Induced Metastasis via the Wnt/-Catenin Pathway in Tongue Squamous Cell Carcinoma. Biomed Res Int. 2017; 2017:6010926. PMC: 5350393. DOI: 10.1155/2017/6010926. View

13.

Udall M, Rizzo M, Kenny J, Doherty J, Dahm S, Robbins P . PD-L1 diagnostic tests: a systematic literature review of scoring algorithms and test-validation metrics. Diagn Pathol. 2018; 13(1):12. PMC: 5807740. DOI: 10.1186/s13000-018-0689-9. View

14.

Tsuji T, Ibaragi S, Hu G . Epithelial-mesenchymal transition and cell cooperativity in metastasis. Cancer Res. 2009; 69(18):7135-9. PMC: 2760965. DOI: 10.1158/0008-5472.CAN-09-1618. View

15.

Vitale M, Cantoni C, Pietra G, Mingari M, Moretta L . Effect of tumor cells and tumor microenvironment on NK-cell function. Eur J Immunol. 2014; 44(6):1582-92. DOI: 10.1002/eji.201344272. View

16.

He Q, Zhou X, Li S, Jin Y, Chen Z, Chen D . MicroRNA-181a suppresses salivary adenoid cystic carcinoma metastasis by targeting MAPK-Snai2 pathway. Biochim Biophys Acta. 2013; 1830(11):5258-66. DOI: 10.1016/j.bbagen.2013.07.028. View

17.

Fang M, Li Y, Huang K, Qi S, Zhang J, Zgodzinski W . IL33 Promotes Colon Cancer Cell Stemness via JNK Activation and Macrophage Recruitment. Cancer Res. 2017; 77(10):2735-2745. PMC: 5760170. DOI: 10.1158/0008-5472.CAN-16-1602. View

18.

Shibutani M, Maeda K, Nagahara H, Fukuoka T, Iseki Y, Matsutani S . Tumor-infiltrating Lymphocytes Predict the Chemotherapeutic Outcomes in Patients with Stage IV Colorectal Cancer. In Vivo. 2017; 32(1):151-158. PMC: 5892643. DOI: 10.21873/invivo.11218. View

19.

Uppendahl L, Felices M, Bendzick L, Ryan C, Kodal B, Hinderlie P . Cytokine-induced memory-like natural killer cells have enhanced function, proliferation, and in vivo expansion against ovarian cancer cells. Gynecol Oncol. 2019; 153(1):149-157. PMC: 6430659. DOI: 10.1016/j.ygyno.2019.01.006. View

20.

Li S, Chien C, Huang W, Luo S, Su Y, Tien W . Prognostic significance and function of mammalian target of rapamycin in tongue squamous cell carcinoma. Sci Rep. 2017; 7(1):8178. PMC: 5558018. DOI: 10.1038/s41598-017-08345-8. View