Integrated Analysis of MiRNA-mRNA Regulatory Network and Functional Verification of MiR-338-3p in Coronary Heart Disease

Overview

Journal Funct Integr Genomics

Publisher Springer

Specialties Genetics
Molecular Biology

Date 2022 Dec 23

PMID 36562844

Authors

Jie Qi

Wenqi Han

Nier Zhong

Qiling Gou

Chaofeng Sun

Affiliations

Soon will be listed here.

Abstract

Coronary heart disease is a cardiovascular disease with high morbidity and mortality. Although great progress has been made in treatment, the prognosis is still very poor. Therefore, this project aims to screen potential diagnostic markers and therapeutic targets related to the progression of coronary heart disease. A total of 94 overlapping differentially expressed mRNAs and 70 differentially expressed miRNAs were identified from GSE20681, GSE12288, GSE49823, and GSE105449. Through a series of bioinformatics methods and experiment, we obtained 5 core miRNA-mRNA regulatory pairs, and selected miR-338-3p/RPS23 for functional analysis. Moreover, we found that RPS23 directly targets miR-338-3p by dual luciferase assay, western, and qPCR. And the expression of miR-338-3p and RPS23 is negatively correlated. The AUC value of miR-338-3p is 0.847. Downregulation of miR-338-3p can significantly inhibit the proliferation and migration of HUVEC. On the contrary, overexpression of miR-338-3p promoted the proliferation and migration of HUVEC. In addition, the interference of RPS23 expression can reverse the regulation of miR-338-3p on HUVEC proliferation. In conclusion, miR-338-3p/RPS23 may be involved in the progression of coronary heart disease, and miR-338-3p may be a diagnostic biomarker and therapeutic target for coronary heart disease.

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References

Ali Syeda Z, Langden S, Munkhzul C, Lee M, Song S . Regulatory Mechanism of MicroRNA Expression in Cancer. Int J Mol Sci. 2020; 21(5). PMC: 7084905. DOI: 10.3390/ijms21051723. View

Caliskan E, de Souza D, Boning A, Liakopoulos O, Choi Y, Pepper J . Saphenous vein grafts in contemporary coronary artery bypass graft surgery. Nat Rev Cardiol. 2019; 17(3):155-169. DOI: 10.1038/s41569-019-0249-3. View

Case B, Waksman R . Coronary Heart Disease: Have We Reached a Plateau in Primary Prevention?. J Am Heart Assoc. 2020; 9(7):e04963. PMC: 7428609. DOI: 10.1161/JAHA.120.016034. View

Fazmin I, Achercouk Z, Edling C, Said A, Jeevaratnam K . Circulating microRNA as a Biomarker for Coronary Artery Disease. Biomolecules. 2020; 10(10). PMC: 7598281. DOI: 10.3390/biom10101354. View

Hong Y, Chen X, Liang Z, Xu Z, Li Y, Pan Y . MiR-338-3p inhibits cell migration and invasion in human hypopharyngeal cancer via downregulation of ADAM17. Anticancer Drugs. 2020; 31(9):925-931. DOI: 10.1097/CAD.0000000000000919. View

Hutter C, Zenklusen J . The Cancer Genome Atlas: Creating Lasting Value beyond Its Data. Cell. 2018; 173(2):283-285. DOI: 10.1016/j.cell.2018.03.042. View

Infante T, Del Viscovo L, De Rimini M, Padula S, Caso P, Napoli C . Network Medicine: A Clinical Approach for Precision Medicine and Personalized Therapy in Coronary Heart Disease. J Atheroscler Thromb. 2019; 27(4):279-302. PMC: 7192819. DOI: 10.5551/jat.52407. View

Katz M, Acevedo J, Wappner P . Growing with the wind. Ribosomal protein hydroxylation and cell growth. Fly (Austin). 2014; 8(3):153-6. PMC: 4594566. DOI: 10.4161/fly.29943. View

Li J, Liu S, Zhou H, Qu L, Yang J . starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res. 2013; 42(Database issue):D92-7. PMC: 3964941. DOI: 10.1093/nar/gkt1248. View

10.

Li L, Mao C, Wang G, Wang N, Xue A . MiR-145-5p alleviates hypoxia/reoxygenation- induced cardiac microvascular endothelial cell injury in coronary heart disease by inhibiting Smad4 expression. Eur Rev Med Pharmacol Sci. 2020; 24(9):5008-5017. DOI: 10.26355/eurrev_202005_21192. View

11.

Liang B, Xiang Y, Zhang X, Wang C, Jin B, Zhao Y . Systematic Pharmacology and GEO Database Mining Revealed the Therapeutic Mechanism of Xuefu Zhuyu Decoration for Atherosclerosis Cardiovascular Disease. Front Cardiovasc Med. 2021; 7:592201. PMC: 7793929. DOI: 10.3389/fcvm.2020.592201. View

12.

Lin J, Jiang J, Zhou R, Li X, Ye J . MicroRNA-451b Participates in Coronary Heart Disease By Targeting VEGFA. Open Med (Wars). 2020; 15:1-7. PMC: 6944456. DOI: 10.1515/med-2020-0001. View

13.

Malakar A, Choudhury D, Halder B, Paul P, Uddin A, Chakraborty S . A review on coronary artery disease, its risk factors, and therapeutics. J Cell Physiol. 2019; 234(10):16812-16823. DOI: 10.1002/jcp.28350. View

14.

Pelliccia F, Pasceri V, Moretti A, Tanzilli G, Speciale G, Gaudio C . Endothelial progenitor cells predict long-term outcome in patients with coronary artery disease: Ten-year follow-up of the PROCREATION extended study. Int J Cardiol. 2020; 318:123-125. DOI: 10.1016/j.ijcard.2020.06.002. View

15.

Pu X, Chan K, Yang W, Xiao Q, Zhang L, Moore A . Effect of a coronary-heart-disease-associated variant of ADAMTS7 on endothelial cell angiogenesis. Atherosclerosis. 2020; 296:11-17. DOI: 10.1016/j.atherosclerosis.2020.01.015. View

16.

Rangel-Zuniga O, Vals-Delgado C, Alcala-Diaz J, Quintana-Navarro G, Krylova Y, Leon-Acuna A . A set of miRNAs predicts T2DM remission in patients with coronary heart disease: from the CORDIOPREV study. Mol Ther Nucleic Acids. 2021; 23:255-263. PMC: 7770508. DOI: 10.1016/j.omtn.2020.11.001. View

17.

Rhee S, Chung J, King D, DAmato G, Paik D, Duan A . Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nat Commun. 2018; 9(1):368. PMC: 5785521. DOI: 10.1038/s41467-017-02796-3. View

18.

Ritchie M, Phipson B, Wu D, Hu Y, Law C, Shi W . limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 2015; 43(7):e47. PMC: 4402510. DOI: 10.1093/nar/gkv007. View

19.

Sherman B, Hao M, Qiu J, Jiao X, Baseler M, Lane H . DAVID: a web server for functional enrichment analysis and functional annotation of gene lists (2021 update). Nucleic Acids Res. 2022; 50(W1):W216-W221. PMC: 9252805. DOI: 10.1093/nar/gkac194. View

20.

Sun F, Yu M, Yu J, Liu Z, Zhou X, Liu Y . miR-338-3p functions as a tumor suppressor in gastric cancer by targeting PTP1B. Cell Death Dis. 2018; 9(5):522. PMC: 5943282. DOI: 10.1038/s41419-018-0611-0. View