Bioinformatics Analysis Identifies Ferroptosis‑related Genes in the Regulatory Mechanism of Myocardial Infarction

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2022 Dec 23

PMID 36561967

Authors

Yong-Hao Jiang

Su-Ying Wu

Zhen Wang

Lei Zhang

Juan Zhang

Yan Li

Chenglong Liu

Wen-Zhe Wu

Yi-Tao Xue

Affiliations

Soon will be listed here.

Abstract

Since ferroptosis is considered to be a notable cause of cardiomyocyte death, inhibiting ferroptosis has become a novel strategy in reducing cardiac cell death and improving cardiopathic conditions. Therefore, the aim of the present study was to search for ferroptosis-related hub genes and determine their diagnostic value in myocardial infarction (MI) to aid in the diagnosis and treatment of the disease. A total of 10,286 DEGs were identified, including 6,822 upregulated and 3.464 downregulated genes in patients with MI compared with healthy controls. After overlapping with ferroptosis-related genes, 128 ferroptosis-related DEGs were obtained. WGCNA successfully identified a further eight functional modules, from which the blue module had the strongest correlation with MI. Blue module genes and ferroptosis-related differentially expressed genes were overlapped to obtain 20 ferroptosis-related genes associated with MI. Go and KEGG analysis showed that these genes were mainly enriched in cellular response to chemical stress, trans complex, transferring, phosphorus-containing groups, protein serine/threonine kinase activity, FoxO signaling pathway. Hub genes were obtained from 20 ferroptosis-related genes through the PPI network. The expression of hub genes was found to be down-regulated in the MI group. Finally, the miRNAs-hub genes and TFs-hub genes networks were constructed. The GSE141512 dataset and the use of RT-qPCR assays on patient blood samples were used to confirm these results. The results showed that ATM, PIK3CA, MAPK8, KRAS and SIRT1 may play key roles in the development of MI, and could therefore be novel markers or targets for the diagnosis or treatment of MI.

Citing Articles

Study on the Synergistic Effect of Klotho and KRAS on Reducing Ferroptosis After Myocardial Infarction by Regulating RAP1/ERK Signaling Pathway.

Cai C, Wu Y, Feng X, Ye X, Liu P, Huang X Appl Biochem Biotechnol. 2025; .

PMID: 39808407 DOI: 10.1007/s12010-024-05171-3.

Transcriptomic analysis and validation study of key genes and the HIF‑1α/HO‑1 pathway associated with ferroptosis in neutrophilic asthma.

Lin L, Liao Z, Li Y, Pan S, Wu S, Sun Q Exp Ther Med. 2024; 28(6):433.

PMID: 39347495 PMC: 11425779. DOI: 10.3892/etm.2024.12722.

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