Comparative Genomic Analysis of a Multidrug-Resistant ShoR14 Clinical Isolate from Terengganu, Malaysia, Led to the Discovery of Novel Mobile Genetic Elements

Overview

Journal Pathogens

Date 2022 Dec 23

PMID 36558739

Authors

Esraa I Al-Trad

Ainal Mardziah Che Hamzah

Suat Moi Puah

Kek Heng Chua

Stephen M Kwong

Chew Chieng Yeo

Ching Hoong Chew

Affiliations

Soon will be listed here.

Abstract

is a coagulase-negative (CoNS) commensal capable of causing serious systemic infections in humans. The emergence of multidrug-resistant strains is of concern but little is known about the characteristics of this organism, particularly from Malaysia. Here, we present the comparative genome analysis of ShoR14, a multidrug-resistant, methicillin-resistant blood isolate from Terengganu, Malaysia. Genomic DNA of ShoR14 was sequenced on the Illumina platform and assembled using Unicycler v0.4.8. ShoR14 belonged to sequence type (ST) 1 which is the most prevalent ST of the subsp. . Comparative genomic analysis with closely related strains in the database with complete genome sequences, led to the discovery of a novel variant of the staphylococcal chromosome cassette (SCC) type VIII element harboring the methicillin-resistance gene in ShoR14 and its possible carriage of a SCC element that encodes the fusidic acid resistance gene (). Up to seven possible ShoR14 plasmid contigs were identified, three of which harbored resistance genes for tetracycline (), chloramphenicol (), macrolides, lincosamides, and streptogramin B (). Additionally, we report the discovery of a novel mercury-resistant transposon, Tn, other genomic islands, and prophages which make up the mobilome.

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