Molecular Networking and Cultivation Profiling Reveals Diverse Natural Product Classes from an Australian Soil-Derived Fungus Sp. CMB-MRF324

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Dec 23

PMID 36558198

Authors

Taizong Wu

Angela A Salim

Paul V Bernhardt

Robert J Capon

Affiliations

Soon will be listed here.

Abstract

This study showcases the application of an integrated workflow of molecular networking chemical profiling (GNPS), together with miniaturized microbioreactor cultivation profiling (MATRIX) to successfully detect, dereplicate, prioritize, optimize the production, isolate, characterize, and identify a diverse selection of new chemically labile natural products from the Queensland sheep pasture soil-derived fungus sp. CMB-MRF324. More specifically, we report the new tryptamine enamino tripeptide aspergillamides E-F (-), dihydroquinoline-2-one aflaquinolones H-I (-), and prenylated phenylbutyrolactone aspulvinone Y (), along with an array of known co-metabolites, including asterriquinones SU5228 () and CT5 (), terrecyclic acid A (), and aspulvinones N-CR (), B (), D (), and H (). Structure elucidation was achieved by a combination of detailed spectroscopic and chemical analysis, biosynthetic considerations, and in the case of an X-ray crystallographic analysis.

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