Hydroethanolic Extract of L. (Stinging Nettle) Leaves As Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 Dec 23

PMID 36558005

Authors

Mohammad A Altamimi

Ibrahim M Abu-Reidah

Almothana Altamimi

Nidal Jaradat

Affiliations

Soon will be listed here.

Abstract

Herbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of (UD) hydroethanolic extract and to screen its antidiabetic activity by disaccharidase hindering and glucose transport in Caco-2 cells. The results have shown that a total of 13 phenolic compounds in this work, viz. caffeic and coumaric acid esters (, , -, ), ferulic derivative (), and flavonoid glycosides (, , -), were identified using HPLC-DAD-ESI/MS. The most abundant phenolic compounds were (rutin) followed by (caffeoylquinic acid III). Less predominant compounds were (caffeoylquinic acid II) and (kaempferol-O-rutinoside). The UD hydroethanolic extract showed 56%, 45%, and 28% (1.0 mg/mL) inhibition level for maltase, sucrase, and lactase, respectively. On the other hand, glucose transport was 1.48 times less at 1.0 mg/mL UD extract compared with the control containing no UD extract. The results confirmed that is a potential antidiabetic herb having both anti-disaccharidase and glucose transport inhibitory properties, which explained the use of UD in traditional medicine.

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