A PPARG Splice Variant in Granulosa Cells Is Associated with Polycystic Ovary Syndrome

Overview

Journal J Clin Med

Specialty General Medicine

Date 2022 Dec 23

PMID 36555903

Authors

Chao-Yi Shi

Jing-Jing Xu

Cheng Li

Jia-Le Yu

Yan-Ting Wu

He-Feng Huang

Affiliations

Soon will be listed here.

Abstract

Background: We explored whether there are splice variants (SVs) of peroxisome proliferator-activated receptor-gamma (PPARG) in polycystic ovary syndrome (PCOS) patients and its relationship with clinical features and KGN cell functions.

Methods: We performed a study involving 153 women with PCOS and 153 age-matched controls. One type of PPARG SV was detected by SMARTer RACE. The correlations between PPARG SV expression levels, clinical features, and KGN cell functions were analyzed. The effect of the PPARG SV on the expression of important genes in metabolism-related pathways was explored by PCR array.

Results: The expression of the PPARG SV in PCOS patients was significantly higher than that in the controls. Clinical features were more significant in the PCOS group with the SV. Compared with overexpression of PPARG, the overexpression of the PPARG SV inhibited the proliferation, migration, and apoptosis of KGN cells in vitro. The genes related to the PPARG SV were mainly involved in lipid metabolism.

Conclusion: While granulosa cells contribute greatly to the development of follicles, our results suggest that the identified PPARG SV may regulate cell proliferation, migration, and apoptosis in granulosa cells, which could partially explain the mechanisms of ovulation dysfunction in PCOS. Further investigation of the utility of this PPARG SV as a biomarker for PCOS is warranted.

Citing Articles

Identification of Novel 58-5p and Interaction and Effects on Apoptosis of Ovine Ovarian Granulosa Cell.

Yang R, Wang Y, Yue S, Liu Y, Zhang Y, Duan C Int J Mol Sci. 2025; 26(2).

PMID: 39859292 PMC: 11765093. DOI: 10.3390/ijms26020576.

The Role of Peroxisome Proliferator-Activated Receptors in Polycystic Ovary Syndrome.

Psilopatis I, Vrettou K, Nousiopoulou E, Palamaris K, Theocharis S J Clin Med. 2023; 12(8).

PMID: 37109247 PMC: 10141215. DOI: 10.3390/jcm12082912.

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