Transcriptional Regulation of Zma- by Action of Nitrate and Auxin in Maize

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Dec 23

PMID 36555358

Authors

Eduardo Lujan-Soto

Paola I Aguirre de la Cruz

Vasti T Juarez-Gonzalez

Jose L Reyes

Maria de la Paz Sanchez

Tzvetanka D Dinkova

Affiliations

Soon will be listed here.

Abstract

In recent years, miR528, a monocot-specific miRNA, has been assigned multifaceted roles during development and stress response in several plant species. However, the transcription regulation and the molecular mechanisms controlling expression in maize are still poorly explored. Here we analyzed the zma- promoter region and found conserved transcription factor binding sites related to diverse signaling pathways, including the nitrate (TGA1/4) and auxin (AuxRE) response networks. Accumulation of both pre-miR528a and mature miR528 was up-regulated by exogenous nitrate and auxin treatments during imbibition, germination, and maize seedling establishment. Functional promoter analyses demonstrated that TGA1/4 and AuxRE sites are required for transcriptional induction by both stimuli. Overall, our findings of the nitrogen- and auxin-induced zma- expression through -regulatory elements in its promoter contribute to the knowledge of miR528 regulome.

Citing Articles

The Fascinating World of Plant Non-Coding RNAs.

Baev V, Gisel A, Minkov I Int J Mol Sci. 2023; 24(12).

PMID: 37373488 PMC: 10299162. DOI: 10.3390/ijms241210341.

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