Marker-Assisted Pyramiding of and Genes to Improve the Clubroot Resistance of

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2022 Dec 23

PMID 36553679

Authors

Xiaonan Li

Yingxia Wei

Yingmei Ma

Guizhu Cao

Siwen Ma

Tianyu Zhang

Zongxiang Zhan

Zhongyun Piao

Affiliations

Soon will be listed here.

Abstract

Clubroot, caused by , is an economically important soil-borne disease that threatens Brassicaceae crops worldwide. In recent years, the incidence area of Chinese cabbage ( ssp. ) clubroot disease has increased, which severely affects the yield and quality of Chinese cabbage. The resistance of varieties harboring the single clubroot-resistance (CR) gene is easily broken through by pathotypes. and , genetically identified in , are CR genes known to be highly resistant to different pathotypes. In our study, we perform the gene pyramiding of and in Chinese cabbages through marker-assisted selection (MAS), and develop homozygous pyramided lines. The newly generated pyramided lines exhibit greater resistance to six different pathotypes than that of two parental lines carrying a single CR gene. This study provides new CR-gene-pyramided lines for the development of clubroot-resistant Brassica varieties for future breeding programs.

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