Insight into Glyproline Peptides' Activity Through the Modulation of the Inflammatory and Neurosignaling Genetic Response Following Cerebral Ischemia-Reperfusion

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2022 Dec 23

PMID 36553646

Authors

Vasily V Stavchansky

Ivan B Filippenkov

Julia A Remizova

Alina E Denisova

Ivan V Mozgovoy

Leonid V Gubsky

Nikolay F Myasoedov

Lyudmila A Andreeva

Svetlana A Limborska

Lyudmila V Dergunova

Affiliations

Soon will be listed here.

Abstract

Glyprolines are Gly-Pro (GP)- or Pro-Gly (PG)-containing biogenic peptides. These peptides can act as neutrophil chemoattractants, or atheroprotective, anticoagulant, and neuroprotective agents. The Pro-Gly-Pro (PGP) tripeptide is an active factor of resistance to the biodegradation of peptide drugs. The synthetic Semax peptide, which includes Met-Glu-His-Phe (MEHF) fragments of adrenocorticotropic hormone and the C-terminal tripeptide PGP, serves as a neuroprotective drug for the treatment of ischemic stroke. Previously, we revealed that Semax mostly prevented the disruption of the gene expression pattern 24 h after a transient middle cerebral artery occlusion (tMCAO) in a rat brain model. The genes of this pattern were grouped into an inflammatory cluster (IC) and a neurotransmitter cluster (NC). Here, using real-time RT-PCR, the effect of other PGP-containing peptides, PGP and Pro-Gly-Pro-Leu (PGPL), on the expression of a number of genes in the IC and NC was studied 24 h after tMCAO. Both the PGP and PGPL peptides showed Semax-unlike effects, predominantly without changing gene expression 24 h after tMCAO. Moreover, there were IC genes (, , and ) for PGP, as well as IC (, , , and ) and NC genes (, , and ) for PGPL, that significantly changed in expression levels after peptide administration compared to Semax treatment under tMCAO conditions. Furthermore, gene enrichment analysis was carried out, and a regulatory gene network was constructed. Thus, the spectra of the common and unique effects of the PGP, PGPL, and Semax peptides under ischemia-reperfusion were distinguished.

Citing Articles

Synthetic Adrenocorticotropic Peptides Modulate the Expression Pattern of Immune Genes in Rat Brain following the Early Post-Stroke Period.

Filippenkov I, Remizova J, Stavchansky V, Denisova A, Gubsky L, Myasoedov N Genes (Basel). 2023; 14(7).

PMID: 37510287 PMC: 10379992. DOI: 10.3390/genes14071382.

Neuroprotective Peptides and New Strategies for Ischemic Stroke Drug Discoveries.

Dergunova L, Filippenkov I, Limborska S, Myasoedov N Genes (Basel). 2023; 14(5).

PMID: 37239313 PMC: 10218113. DOI: 10.3390/genes14050953.

Special Issue "Genomics of Stroke" 2022.

Limborska S, Filippenkov I Genes (Basel). 2023; 14(2).

PMID: 36833441 PMC: 9956966. DOI: 10.3390/genes14020514.

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